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Research Article | Open Access

Bioactivity Behaviour of Osteoblasts on Commercially Pure Titanium with TiO2-ZrO2 Nanocomposite Mixture Coating

Thekra Ismael Hamad1( ), Rawaa Zaher Hassan Zwain2, Ali Mohammad Ali Aljafery2
Department of Prosthodontics, College of Dentistry, University of Baghdad, Iraq
Department of Prosthodontics, College of Dentistry, University of Kufa, Iraq
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Abstract

This study aims to improve the bioactivity response of osteoblasts attachment and proliferation on commercial pure titanium surface by the mixture of nanocomposite coating material of 70% TiO2 (rutile 10-30 nm, Sky Spring Nanomaterials, USA) and 30% ZrO2 (20-30 nm, Sky Spring Nanomaterials, USA). This may increase the likelihood of developing the modified implant surface by electrophoretic deposition and the dipping methods of nanocomposite mixture to enhance the surface bioactivity and promote bone formation. Three groups of commercially pure titanium: one uncoated group and two coated groups with nanocomposite mixture of different coating techniques; one group for electro photic deposition technique (EPD); and one group for dip technique. The bioactivity evaluation of cell cultures, isolation of osteoblast cells from calvaria and long limbs of 3-4 days neonatal rats to evaluate the attachment and the proliferation assay in 4 and 8 days of incubation periods for each group. However, osteoblast cells attachment and proliferation showed the least attached and proliferated cells in uncoated samples, while the EPD coated sample showed the highest.

Keywords

NanocompositeCommercially pure titaniumOsteoblastsElectrophoretic depositionDip technique

References

[1]

I. Abrahamsson, G. Cardaropoli, Peri‐implant hard and soft tissue integration to dental implants made of titanium and gold. Clinical Oral Implants Research, 2007, 18(3): 269-274.
Crossref Google Scholar
[2]

M. Saini, Y. Singh, P. Arora, et al., Implant biomaterials: A comprehensive review. World Journal of Clinical Cases: WJCC, 2015, 3(1): 52.
Crossref Google Scholar
[3]

F. Rupp, L. Liang, J. Geis-Gerstorfer, et al., Surface characteristics of dental implants: A review. Dental Materials, 2018, 34(1): 40-57.
Crossref Google Scholar
[4]

P. Mandracci, F. Mussano, P. Rivolo, et al., Surface treatments and functional coatings for biocompatibility improvement and bacterial adhesion reduction in dental implantology. Coatings, 2016, 6(1): 7.
Crossref Google Scholar
[5]

R.Z.H. Zwain, T.I. Hamad, Coating evaluation of nanocomposite mixture of TiO2 and ZrO2 by electrophoretic deposition and dip techniques on commercially pure titanium. Journal of Research in Medical and Dental Science, 2018, 6(2): 483.
Google Scholar
[6]
Z.C. Wang, Y.J. Ni, and J.C. Huang, Fabrication and characterization of HAp/Al2O3 composite coating on titanium substrate. Proceedings of the 2nd IEEE International Conference on Bioinformatics and Biomedical Engineering. 2008: 1526-1530.
Crossref
[7]

S. Anil, P.S. Anand, H. Alghamdi, et al., Dental implant surface enhancement and osseointegration. Implant dentistry-a rapidly evolving practice, 2011: 83-108.
Crossref Google Scholar
[8]

J. Si, J. Zhang, S. Liu, et al., Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro. Acta Biochim Biophys Sin, 2014, 46(7): 572-581.
Crossref Google Scholar
[9]

S.E.B. Taylor, M. Shah, and I.R. Orriss, Generation of rodent and human osteoblasts. BoneKEy Reports, 2014, 3.
Crossref Google Scholar
[10]

Z.G.M. Azzawi, T.I. Hamad, S.A. Kadhim, et al., Osseointegration evaluation of laser-deposited titanium dioxide nanoparticles on commercially pure titanium dental implants. Journal of Materials Science: Materials in Medicine, 2018, 29(7): 96.
Crossref Google Scholar
[11]
J.P. Dillon, V.J. Waring-Green, A.M. Taylor, et al., Primary human osteoblast cultures. Bone research protocols. Humana Press, 2012: 3-18.
Crossref
[12]

A.L. Rosa, M.M. Beloti, Effect of cpTi surface roughness on human bone marrow cell attachment, proliferation, and differentiation. Brazilian Dental Journal, 2003, 14(1): 16-21.
Crossref Google Scholar
[13]

C.A. Gregory, W.G. Gunn, A. Peister, et al., An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Analytical Biochemistry, 2004, 329(1): 77-84.
Crossref Google Scholar
[14]

M. Jäger, C. Zilkens, K. Zanger, et al., Significance of nano-and microtopography for cell-surface interactions in orthopaedic implants. BioMed Research International, 2007, 2007.
Crossref Google Scholar
[15]
S. Basu, J. Wang, and A. Paul, Commentary 2 nanomaterials for bone repair. 2017.
[16]

T.J. Webster, C. Ergun, R.H. Doremus, et al., Increased osteoblast adhesion on titanium‐coated hydroxylapatite that forms CaTiO3. Journal of Biomedical Materials Research Part A, 2003, 67(3): 975-980.
Crossref Google Scholar
[17]

J. Marchi, V. Ussui, C.S. Delfino, et al., Analysis in vitro of the cytotoxicity of potential implant materials. Ⅰ: Zirconia‐titania sintered ceramics. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2010, 94(2): 305-311.
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 11 Issue 4,
December 2019
Pages 361-367
DOI: 10.5101/nbe.v11i4.p361-367
Cite this article:
Hamad TI, Zwain RZH, Aljafery AMA. Bioactivity Behaviour of Osteoblasts on Commercially Pure Titanium with TiO2-ZrO2 Nanocomposite Mixture Coating. Nano Biomedicine and Engineering, 2019, 11(4): 361-367. https://doi.org/10.5101/nbe.v11i4.p361-367

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Received: 01 August 2019
Accepted: 20 November 2019
Published: 20 November 2019
© Thekra Ismael Hamad, Rawaa Zaher Hassan Zwain, and Ali Mohammad Ali Aljafery.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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