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Research Article | Open Access

Characterization and Efficacy of A Novel Formula of Insulin Chitosan Layered Duple Hydroxide-Nanohybrid for Oral Administration

Ahmed Abdel-Wahab El-Shahawy1, Adel Abdel-Moneim2( ), Zienab Esam Eldin3, Ahmed Ismail Youssef2, Zhraa Selem Gouda2, Hanaa Ramadan2, Eiman Amar2, Esraa Ashraf Taha2, Asmaa Mohamed Saied2, Mohamed Zanaty4
Department of Materials Science and Nanotechnology, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Egypt
Division of Molecular Physiology, Faculty of Science, Beni-Suef University, Egypt
Faculty of Veterinary Medicine, Beni-Suef University, Egypt
Department of Biotechnology, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Egypt
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Abstract

The development of an oral insulin therapy remains an ultimate goal to both enhance ease of use, and to provide therapeutic advantages rooted in its direct delivery to the portal vein and liver. The current study aimed to develop a novel formula for insulin oral administration against experimental diabetes in rats. Entrapped insulin (INS) between chitosan nanoparticles (CSNPs) and layered duple hydroxide (LDH) (LDH-INS-CSNPs) was chemically prepared, and characterized by X-ray diffraction, Fourier transformation infrared, high-resolution transmission electron microscope, field emission scanning electron microscope and Zeta potential measurements. The insulin release study was conducted in vitro, while the oral hypoglycemic effect of LDH-INS-CSNPs was investigated in vivo in diabetic rats. The prepared formulas revealed a variation in the spectra of characterization methods. The insulin entrapment between LDH and chitosan avoided the burst release of insulin and acid denaturation in the stomach and enzymatic degradation throughout the gut. Consequently, the blood glucose level of LDH-INS-CSNPs formula exhibited a marked hypoglycemic effect. The present work showed that the LDH-INS-CSNPs formula had a protective effect against enzymatic degradation, reduced insulin initial burst release, and enhanced bioavailability through the oral administration route. Interestingly, the presented formula could be an oral antidiabetic agent alternative to injectable insulin.

Keywords

DiabetesInsulinOral administrationBioavailabilitySustain release

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Nano Biomedicine and Engineering
Volume 12 Issue 4,
December 2020
Pages 297-305
DOI: 10.5101/nbe.v12i4.p297-305
Cite this article:
Abdel-Wahab El-Shahawy A, Abdel-Moneim A, Esam Eldin Z, et al. Characterization and Efficacy of A Novel Formula of Insulin Chitosan Layered Duple Hydroxide-Nanohybrid for Oral Administration. Nano Biomedicine and Engineering, 2020, 12(4): 297-305. https://doi.org/10.5101/nbe.v12i4.p297-305

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Received: 23 May 2020
Accepted: 01 September 2020
Published: 23 October 2020
© Ahmed El-Shahawy, Adel Abdel-Moneim, Zienab Eldin, Ahmed Youssef, Zhraa Gouda, Hanaa Ramadan, Eiman Amar, Esraa Taha, Asmaa Saied, and Mohamed Zanaty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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