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Research Article | Open Access

Assessing the Effects of Administering Calcium Oxide Nanoparticles on Oral Mucosa and Tongue of Male Wister Rats

Bushra Habeeb Al-Maula1, Zena Wally2(), Rasha Dosh3, Abtesam Imhemed Aljdaimi4, Suhad Jabbar Hamed Al-Nasrawie5, Julfikar Haider6
Scientific Center of Laser and Photonics, University of Al-Hamdaniya, Ninvah, Iraq
Department of Prosthodontic, Faculty of Dentistry, University of Kufa, Najaf, Iraq
Department of Anatomy and Histology, Faculty of Medicine, University of Kufa, Najaf, Iraq
College of Dentistry and Oral Surgery, Alasmarya University, Libya
Department of Conservative Dentistry, Faculty of Dentistry, University of Kufa, Iraq
Department of Engineering, Manchester Metropolitan University, Manchester, UK
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Abstract

The present study was conducted to evaluate the impact of calcium oxide-nanoparticles (CaO-NPs) administration at different concentrations on the epithelial thickness, papilla length and number of blood vessels of male Wister rats' buccal mucosa, and the epithelial thickness, filiform length and number of blood vessels of the rats' tongue. Twelve rats were used and randomly allocated into four groups: control group (untreated) and the three experimental groups were orally treated with CaO-NPs at different doses 25, 50 and 100 mg/kg of the body weight over two months. On the 61st day, all the animals were sacrificed, and 1 cm of the buccal mucosa and the body of the tongue were carefully removed for histological analysis. Histological tissues were studied under a light microscope to investigate the impact of CaO-NPs administration on the oral tissues. An image processing software (Image J) was used to measure the epithelial thickness, papilla length and number of blood vessels of the rats' buccal mucosa, and the epithelial thickness, filiform length and number of blood vessels of the rats' tongue. The results showed that the CaO nanoparticles administration caused epithelial atrophy and decreased vascularization of buccal and tongue mucosa with all tested doses of CaO-NPs and the 100 mg/kg concentration showed the most significant effect.

Keywords

CaO nanoparticlesDrug administrationOral mucosaTongueHistological analysis

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Nano Biomedicine and Engineering
Volume 13 Issue 3,
September 2021
Pages 311-320
DOI: 10.5101/nbe.v13i3.p311-320
Cite this article:
Al-Maula BH, Wally Z, Dosh R, et al. Assessing the Effects of Administering Calcium Oxide Nanoparticles on Oral Mucosa and Tongue of Male Wister Rats. Nano Biomedicine and Engineering, 2021, 13(3): 311-320. https://doi.org/10.5101/nbe.v13i3.p311-320
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