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Article | Open Access

The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen

Dongwu Liu1,2( ), Zhiwei Chen1,2, Xue Wang2, Hongzhi Xu1, Lina Wang1
Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong Province 255049
School of Life Sciences, Shandong University of Technology, Zibo, Shandong Province 255049
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Abstract

Confocal microscope, which is a major advance upon normal light microscope, has been used in a number of scientific fields. Moreover, the three-dimensional images of specimens can be reconstructed with confocal microscope. It is ideal to analyze the three dimensional specimens for the non-destructive, non-invasive nature of the confocal microscope. In the present studies, a series of Weigela floridacv and Lonicera japonica thunb pollen optical sections were acquired with confocal microscope. Then the three-dimensional images of the pollen were reconstructed with the software of confocal microscope. In addition, intracellular calcium in the pollens was detected with the probe Fluo-3 AM, and the distribution of calcium in the pollens was analyzed with confocal microscope. Our results indicate that it is a very easy job to analyze the three-dimensional digital images of the pollen and intracellular calcium in the pollens with confocal microscope and the probes Acridine orange (AO) and Fluo-3 AM.

Keywords

ReconstructionCalciumPollenConfocal microscopeThree-dimensional image

References

[1]

Lichtman JW. Confocal microscopy. Scientific American 1994; 271: 40–53. doi: 10.1038/scientificamerican0894-40
Crossref Google Scholar
[2]

Hepler PK, Vidali L, Cheung AY. Polarized cell growth in higher plants. Annu Rev Cell Dev Biol 2001; 17: 159–187. doi: 10.1146/annurev.cellbio.17.1.159
Crossref Google Scholar
[3]

Yamaoka S, Leaver CJ. EMB2473/MIRO1, an Arabidopsis Miro GTPase, is required for embryogenesis and influences mitochondrial morphology in pollen. Plant Cell 2008; 20: 589–601. doi: 10.1105/tpc.107.055756
Crossref Google Scholar
[4]

Liu ZQ, Xu GH, Zhang SL. Pyrus pyrifolia stylar S-RNase induces alterations in the actin cytoskeleton in self-pollen and tubes in vitro. Protoplasma 2007; 232: 61–67. doi: 10.1007/s00709-007-0269-4
Crossref Google Scholar
[5]

Roy SJ, Holdaway-Clarke TL, Hackett GR, Kunkel JG, Lord EM, Hepler PK. Uncoupling secretion and tip growth in lily pollen tubes: evidence for the role of calcium in exocytosis. Plant J 1999; 19: 379–386. doi: 10.1046/j.1365-313X.1999.00515.x
Crossref Google Scholar
[6]

Feijó JA, Malho' R, Obermeyer G. Ion dynamics and its possible role during in vitro pollen germination and tube growth. Protoplasma 1995; 187: 155–167. doi: 10.1007/BF01280244
Crossref Google Scholar
[7]

Tayler LP, Hepler PK. Pollen germination and tube growth. Annu Rev Plant Physiol Plant Mol Biol 1997; 48: 461–491. doi: 10.1146/annurev.arplant.48.1.461
Crossref Google Scholar
[8]

Bove J, Vaillancourt B, Kroeger J, Hepler PK, Wiseman PW, Geitmann A. Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching. Plant Physiol 2008; 147: 1646–1658. doi: 10.1104/pp.108.120212
Crossref Google Scholar
[9]

Dinsmore AD, Weeks ER, Prasad V, Levitt AC, Weitz DA. Three-Dimensional Confocal Microscopy of Colloids. Applied optics 2001; 40: 4152–4159. doi: 10.1364/AO.40.004152
Crossref Google Scholar
[10]

Van der Voort HT, Brankenhoff GJ, Baarlslag MW. Three-dimensional visualization methods for confocal microscopy. J Microsc 1989; 153: 123–132.
Crossref Google Scholar
[11]

McCauley MM, Hepler PK. Cortical ultrastructure of freeze-substituted protonemata of the moss Funaria hygrometrica. Protoplasma 1992; 169: 215–230. doi: 10.1007/BF01323616
Crossref Google Scholar
[12]

Gray JD, Kolesik P, Hoj PB, Coombe BG. Confocal measurement of the three-dimensional size and shape of plant parenchyma cells in a developing fruit tissue. Plant J 1999; 19: 229–236. doi: 10.1046/j.1365-313X.1999.00512.x
Crossref Google Scholar
[13]

Funada R, Abe H, Furusawa O, Imaizumi H, Fukazawa K and Ohtani J. The orientation and localization of cortical microtubules in differentiating conifer tracheids during cell expansion. Plant Cell Physiol 1997; 38: 210–212.
Crossref Google Scholar
[14]

Kitin P, Funada R, Sano Y, Ohtani J. Analysis by Confocal Microscopy of the Structure of Cambium in the Hardwood Kalopanax pictus. Ann Bot 2000; 86: 1109–1117. doi: 10.1006/anbo.2000.1281
Crossref Google Scholar
[15]

Kitin P, Sano Y, Funada R. Fusiform cells in the cambium of Kalopanax pictus are exclusively mononucleate. J Exp Bot 2002; 53: 483–488. doi: 10.1093/jexbot/53.368.483
Crossref Google Scholar
[16]

Kao JP, Harootunian AT, Tsien RY. Photochemically generated cytosolic calcium pulses and their detection by fluo-3. J Biol Chem 1989; 264: 8179–8184.
Crossref Google Scholar
[17]

Minta A, Kao JP, Tsien RY. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 1989; 264: 8171–8178.
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 1 Issue 1,
March 2009
Pages 57-60
DOI: 10.5101/nbe.v1i1.p57-60
Cite this article:
Liu D, Chen Z, Wang X, et al. The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen. Nano Biomedicine and Engineering, 2009, 1(1): 57-60. https://doi.org/10.5101/nbe.v1i1.p57-60

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Received: 10 November 2009
Accepted: 06 December 2009
Published: 09 December 2009
© 2009 D. Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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