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Article | Open Access

Physical-chemical Properties and in vitro Biocompatibility Assessment of Spider Silk, Collagen and Polyurethane Nanofiber Scaffolds for Vascular Tissue Engineering

Chuanglong He1,2( ), Lei Zhang2, Hongsheng Wang2, Fan Zhang2, Xiumei Mo1,2
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 2016204
College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University,Shanghai, 201620
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Abstract

In this study, three kinds of nanofiber scaffolds including spider silks (SS), collagen, and polyurethane (PU) were fabricated by electrospinning technique. Their physical-chemical properties such as surface hydrophilicity, water stability, and porosity were investigated by water contact angle (CA) measurement, stabilization assay and scanning electron microscope (SEM). Results showed that SS scaffolds had stronger hydrophobic surface, superior water-stability and higher porosity than other scaffolds. Furthermore, their in vitro biocompatibility including cell attachment, spreading, and proliferation were evaluated and compared by using porcine aorta endothelium cells (PIECs). The MTT results showed that the cell proliferation on SS nanofibers was significantly higher than that on collagen and PU scaffoldss, the SEM images demonstrated that the PIECs can migrate into SS nanofibers and maintain a spreading shape, and the RT-PCR results also indicated the SS nanofiber scaffolds promote better cell growth and proliferation. Thus, these results strongly suggest the potential application of SS nanofibers as vascular engineering scaffolds.

Keywords

Spider silkIn Vitro BiocompatibilityElectrospinningNanofibersVascular tissue engineering

References

[1]

Grenier S, Sandig M, Mequanint K. Polyurethane biomaterials for fabricating 3D porous scaffolds and supporting vascular cells. J Biomed Mater Res 2007; 82 A: 802-9.
Crossref Google Scholar
[2]

Arrigoni C, Camozzi D, Remuzzi A. Vascular tissue engineering. Cell Transplant 2006; 15: S119-S25. doi: 10.3727/000000006783982430
Crossref Google Scholar
[3]

Nerem RM, Seliktar D. Vascular tissue engineering. Annu Rev Biomed Eng 2001; 3: 225-43. doi: 10.1146/annurev.bioeng.3.1.225
Crossref Google Scholar
[4]

Jeong SI, Kim SH, Kim YH, Jung Y, Kwon JH, Kim BS. Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering. J Biomater Sci Polym Ed 2004; 15: 645-60. doi: 10.1163/156856204323046906
Crossref Google Scholar
[5]

Williamson MR, Black R, Kielty C. PCL-PU composite vascular scaffold production for vascular tissue engineering: Attachment, proliferation and bioactivity of human vascular endothelial cells. Biomaterials 2006; 27: 3608-16.
Crossref Google Scholar
[6]

Patel HJ, Su SH, Patterson C, Nguyen KT. A combined strategy to reduce restenosis for vascular tissue engineering applications. Biotechnol Prog 2006; 22: 38-44. doi: 10.1021/bp050135e
Crossref Google Scholar
[7]

Higgins SP, Solan AK, Niklason LE. Effects of polyglycolic acid on porcine smooth muscle cell growthand differentiation. J Biomed Mater Res 2003; 67A: 295-302. doi: 10.1002/jbm.a.10599
Crossref Google Scholar
[8]

Zhu CH, Fan DD, Duan ZZ, Xue WJ, Shang LA, Chen FL. Initial investigation of novel human-like collagen/chitosan scaffold for vascular tissue engineering. J Biomed Mater Res A 2009; 89A: 829-40. doi: 10.1002/jbm.a.32256
Crossref Google Scholar
[9]

Zorlutuna P, Hasirci N, Hasirci V. Nanopatterned collagen tubes for vascular tissue engineering. J Tissue Eng Regen Med 2008; 2: 373-7. doi: 10.1002/term.99
Crossref Google Scholar
[10]

Shaikh FM, Brien TPO, Callanan A, Kavanagh EG, Burke PE, Grace PA. Fibrin gel scaffold optimization for tissue engineering vascular grafts. Tissue Eng 2007; 13: 186.
Google Scholar
[11]

Shaikh FM, Callanan A, Kavanagh EG, Burke PE, Grace PA, McGloughlin TM. Fibrin: A natural biodegradable scaffold in vascular tissue engineering. Cells Tissues Organs 2008; 188: 333-46. doi: 10.1159/000139772
Crossref Google Scholar
[12]

Yao L, Swartz DD, Gugino SF, Russell JA, Andreadis ST. Fibrin-based tissue-engineered blood vessels: Differential effects of biomaterial and culture parameters on mechanical strength and vascular reactivity. Tissue Eng 2005; 11: 991-1003. doi: 10.1089/ten.2005.11.991
Crossref Google Scholar
[13]

L'Heureux N, Paquet S, Labbe R, Germain L, Auger FA. A completely biological tissue-engineered human blood vessel. Faseb J 1998; 12: 47-56.
Crossref Google Scholar
[14]

Boland ED, Matthews JA, Pawlowski KJ, Simpson DG, Wnek GE, Bowlin GL. Electrospinning collagen and elastin: Preliminary vascular tissue engineering. Front Biosci 2004; 9: 1422-32. doi: 10.2741/1313
Crossref Google Scholar
[15]

Yoo JJ, Liu J, Soker S, Komura M, Lim G, Atala A. Electrospinning fabrication of collagen-based scaffolds for vascular tissue engineering. Faseb J 2006; 20: A1101-A.
Crossref Google Scholar
[16]

Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 2003; 63: 2223-53. doi: 10.1016/S0266-3538(03)00178-7
Crossref Google Scholar
[17]

Ma PX. Biomimetic materials for tissue engineering. Adv Drug Deliv Rev 2008; 60: 184-98.
Crossref Google Scholar
[18]

He CL, Huang ZM, Han XJ. Fabrication of drug-loaded electrospun aligned fibrous threads for suture applications. J Biomed Mater Res 2009; 89A: 80-95. doi: 10.1002/jbm.a.32004
Crossref Google Scholar
[19]

Xu CY, Inai R, Kotaki M, Ramakrishna S. Aligned biodegradable nanotibrous structure: a potential scaffold for blood vessel engineering. Biomaterials 2004; 25: 877-86. doi: 10.1016/S0142-9612(03)00593-3
Crossref Google Scholar
[20]

Stephens JS, Fahnestock SR, Farmer RS, Kiick KL, Chase DB, Rabolt JF. Effects of electrospinning and solution casting protocols on the secondary structure of a genetically engineered dragline spider silk analogue investigated via fourier transform Raman spectroscopy. Biomacromolecules 2005; 6: 1405-13.
Crossref Google Scholar
[21]

Zhou SB, Peng HS, Yu XJ, Zheng XT, Cui WG, Zhang ZR, et al. Preparation and characterization of a novel electrospun spider silk fibroin/poly(D,L-lactide) composite fiber. J Phys Chem B 2008; 112: 11209-16. doi: 10.1021/bm049296hdoi:10.1021/jp800913k
Crossref Google Scholar
[22]

Zarkoob S, Eby RK, Reneker DH, Hudson SD, Ertley D, Adams WW. Structure and morphology of electrospun silk nanofibers. Polymer 2004; 45: 3973-7.
Crossref Google Scholar
[23]

Huang ZM, Zhang YZ, Ramakrishna S, Lim CT. Electrospinning and mechanical characterization of gelatin nanofibers. Polymer 2004; 45: 5361- 8. doi: 10.1016/j.polymer.2003.10.102
Crossref Google Scholar
[24]

Work R, Young C. The amino acid compositions of major and minor ampullate silks of certain orb-web-building spiders (Araneae, Araneidae). J Arachnol 1987: 65-80. doi: 10.1016/j.polymer.2004.04.005
Crossref Google Scholar
[25]

Walrafen G, Chu Y. Nature of collagen-water hydration forces: A problem in water structure. Chem Phys 2000; 258: 427-46. doi: 10.1016/S0301-0104(00)00072-0
Crossref Google Scholar
[26]

Sirichaisit J, Brookes V, Young R, Vollrath F. Analysis of structure/property relationships in silkworm (Bombyx mori) and spider dragline (Nephila edulis) silks using Raman spectroscopy. Biomacromolecules 2003; 4: 387-94. doi: 10.1021/bm0256956
Crossref Google Scholar
[27]

Zong X, Kim K, Fang D, Ran S, Hsiao B, Chu B. Structure and process relationship of electrospun bioabsorbable nanofiber membranes. Polymer 2002; 43: 4403-12. doi: 10.1016/S0032-3861(02)00275-6
Crossref Google Scholar
[28]

Tamada Y, Ikada Y. Fibroblast growth on polymer surfaces and biosynthesis of collagen. J Biomed Mater Res 1994; 28: 783-90. doi: 10.1002/jbm.820280705
Crossref Google Scholar
[29]

Dee KC, Puleo DA, Bizios R. Blood-Biomaterial Interactions and Coagulation. An introduction to tissue¨Cbiomaterial interactions, Wiley, New York. 2002: 37¨C87.
Crossref
Nano Biomedicine and Engineering
Volume 1 Issue 1,
March 2009
Pages 80-88
DOI: 10.5101/nbe.v1i1.p80-88
Cite this article:
He C, Zhang L, Wang H, et al. Physical-chemical Properties and in vitro Biocompatibility Assessment of Spider Silk, Collagen and Polyurethane Nanofiber Scaffolds for Vascular Tissue Engineering. Nano Biomedicine and Engineering, 2009, 1(1): 80-88. https://doi.org/10.5101/nbe.v1i1.p80-88

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Received: 10 November 2009
Accepted: 06 December 2009
Published: 09 December 2009
© 2009 C. He et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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