Abstract
Paclitaxel is routinely used in cancer chemotherapy to treat patients with ovarian, breast, lung, head and neck cancers. However, because of its low aqueous solubility, it’s been administered as a Cremophor EL and ethanol solution, which is associated with increased toxicity and high therapeutic dose requirements. The goal of the present study was to formulate paclitaxel into a self assembling nanoemulsion (SANE) and demonstrate the effects of paclitaxel SANE formulation on the inhibition of cell proliferation in breast (80 %), colon (60 %), and pancreatic cell lines (60 %) compared to blank nanoemulsion. In addition, nanoemulsions of paclitaxel with a mean particle size of 20 nm dramatically reduced zeta potential and showed up to 12 fold greater apoptosis in the PL-45 pancreatic cancer cell line compared to a blank nanoemulsion. In conclusion we have developed a SANE formulation of paclitaxel having a particle size of 20 nm which significantly inhibited cell proliferation, dramatically reduced zeta potential and increased apoptosis by 12-fold when compared to a blank and nanoemulsion, thus indicating the therapeutic potential for SANE as an anti-cancer drug delivery system.