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Article | Open Access

Synthesis of Ru(bpy)3-doped Silica Nanoparticle and Its Application in Fluorescent Immunoassay

Dongguang Yin1( ), Binhu Liu, Li Zhang, Chunjuan Xie, Le Zhang
College of Environmental and Chemical Engineering, Shanghai University, Shanghai
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Abstract

A novel type of amino functionalized core-shell Ru(bpy)3-doped silica nanoparticles was synthesized using a simple and effective approach of reverse microemulsion. The nanoparticles were characterized by transmission electron microscope, fluorescence spectra, UV-Vis spectroscopy and tests of photostability and dye molecular leakage. It was found that the nanoparticles exhibit excellent fluorescent properties such as extremely bright, highly photostable and chemicalstable. Furthermore, the nanoparticles utilized as a fluorescent marker applying in fluorescent immunoassay of mouse IgG were studied and desired results were obtained.

Keywords

Silica nanoparticlesImmunoassayRu(bpy)3-dopedReverse microemulsion

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Nano Biomedicine and Engineering
Volume 2 Issue 2,
June 2010
Pages 117-120
DOI: 10.5101/nbe.v2i2.p117-120
Cite this article:
Yin D, Liu B, Zhang L, et al. Synthesis of Ru(bpy)3-doped Silica Nanoparticle and Its Application in Fluorescent Immunoassay. Nano Biomedicine and Engineering, 2010, 2(2): 117-120. https://doi.org/10.5101/nbe.v2i2.p117-120

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Received: 26 May 2010
Accepted: 10 June 2010
Published: 26 June 2010
© 2010 D. Yin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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