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Article | Open Access

Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

Daxiang Cui1,2( ), Hong Zhang2, Jie Sheng3, Zheng Wang3, Asahi Toru2, Rong He1, Osaka Tetsuya2, Feng Gao1, Hoon Sung Cho4, Chris Huth4, Hengyao Hu1, Giovanni M. Pauletti5, Donglu Shi1,4
Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, China
Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, Japan
Stem Cell Lab., Institute of Digestion Diseases, RenJi Hospital, Shanghai JiaoTong University, Shandong Road, Shanghai20001, China
Department of Chemical and Materials Engineering, University of Cincinnati, Cincinnati, OH 45221, USA
James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267, USA
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Abstract

Stem cells nanotechnology has emerged as a new exciting area, and holds great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine. Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy (HR-TEM), and incubated with murine ES CCE cells for 1 to 28 day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction (RTPCR), flow cytometry (FCM) and MTT method. Results showed that prepared FMNTs exhibited green fluorescent signal, could enter into ES cells in time-dependent means, more than 20 µg ml-1 FMNTs induced ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose-and time-dependent means, induced apoptosis of ES cells; conversely, 5 µg ml-1 FMNTs could markedly stimulate the expression of Sox1 and Hsp27, and inhibit expression of OCT4 in ES cells, FCM analysis showed that differentiation marker Flk-1 exhibited higher expression compared with control ES cells. In conclusion, high dose of FMNTs can inhibit proliferation of ES cells, low dose of FMNTs can improve the differentiation of ES cells, FMNTs can have potential applications in in vivo tracking, imaging and regulation of the proliferation and differentiation of ES cells.

Keywords

proliferationdifferentiationmulti-walled carbon nanotubesapoptosisCdSe/ZnS quantum dotsmurine embryonic stem cells

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Nano Biomedicine and Engineering
Volume 2 Issue 4,
December 2010
Pages 236-244
DOI: 10.5101/nbe.v2i4.p236-244
Cite this article:
Cui D, Zhang H, Sheng J, et al. Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells. Nano Biomedicine and Engineering, 2010, 2(4): 236-244. https://doi.org/10.5101/nbe.v2i4.p236-244

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Received: 10 November 2010
Accepted: 06 December 2010
Published: 16 December 2010
© 2010 D. Cui, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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