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Article | Open Access

Time-Resolved Fluorescence Immunoassay of Mouse IgG using Europium(Ш) Chelate-doped Silica Nanoparticles

Dongguang Yin( ), Li Zhang, Binhu Liu, Le Zhang, Hu Yan
College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, P. R. China
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Abstract

Nanoparticle labes conjuaged with biomolecules have been used in a variety of different assay application. We investigated the possibility of using europium(Ⅲ) chated-doped silica nanoparticle conjugated with streptavidin (SA) to detect mouse IgG by time-resolved fluorescence immunoassay (TRFIA). Results demonstrate that the nanoparticle is a novel kind of superior fluourscent probe which could be effectively applied in time-resolved fluorescent immunoassay with high detective sensitivity. In the present study, the lowest detection limit of mouse IgG is 34 pg mL-1.

Keywords

Silica nanoparticleFluorescent probeEuropium chelateTime-resolved fluorescenceMouse IgG

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Nano Biomedicine and Engineering
Volume 3 Issue 1,
March 2011
Pages 25-28
DOI: 10.5101/nbe.v3i1.p25-28
Cite this article:
Yin D, Zhang L, Liu B, et al. Time-Resolved Fluorescence Immunoassay of Mouse IgG using Europium(Ш) Chelate-doped Silica Nanoparticles. Nano Biomedicine and Engineering, 2011, 3(1): 25-28. https://doi.org/10.5101/nbe.v3i1.p25-28

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Published: 31 March 2011
© 2011 D. Yin, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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