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Article | Open Access

Activated Carbon Nanoparticles As Carriers of Anticancer Drugs

Lan Sun1, Yaoxin Cai2, Yanlei Liu1, Dongying Song3, Yan Liu1, Hongjuan Yao1, Yingge Zhang1( )
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing100850, China
Department of Pharmacy, Chinese PLA General Hospital, Beijing100853, China
Jilin University, Changchun 130021, China
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Abstract

The potentials of activated carbon nanoparticles (ACNP) as anticancer drug carriers were studied. ACNP were prepared with a top-down method. ACNP-based drug delivery system of docetaxel (ACNP-DOC) was prepared with a simple absorption method and its effects were studied primarily with methyl thiazolyl tetrazolium (MTT) assay, light microscope (LM), atomic force microscope (AFM) and transmission electron microscope (TEM). The prepared ACNP were approximately globular in shape with an average size of 233 nm, which had a saturate adsorption of 195.69 mg/g at mass ratio of ACNP: DOC=5:1. The drug delivery system prepared by adsorbing of ACNP for DOC had excellent releasing profile in the simulated in vivo environment. LM observation showed that ACNP can accumulate around the cells and on the surface of cells and TEM revealed that ACNP can enter cells and nuclears. MTT test demonstrated that ACNP-DOC had inhibitive effects on the growth of A549 cells, with an IC50 of 0.79 μg/ml, significantly smaller than that of 20 μg/ml of free DOC, indicating ACNP-DOC had stronger effects than free DOC. The imaging of AFM showed that both DOC and ACNP-DOC caused significant pathological changes of the cell membrane, including rough surfaces, large grains and holes, but these pathological changes were more obvious in ACNP-DOC treated cells than those free DOC treated ones. In all of the experiments, ACNP themselves had no significant influences on A549 cells. It was concluded that ACNP could serve as a good nanocarrier for anti-cancer drug delivery to target cells and have a great potential application in antitumor chemotherapy.

Keywords

Atomic force microscopyA549 cellsActivated carbon nanoparticlesDocetaxel

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Nano Biomedicine and Engineering
Volume 5 Issue 2,
June 2013
Pages 94-101
DOI: 10.5101/nbe.v3i1.p94-101
Cite this article:
Sun L, Cai Y, Liu Y, et al. Activated Carbon Nanoparticles As Carriers of Anticancer Drugs. Nano Biomedicine and Engineering, 2013, 5(2): 94-101. https://doi.org/10.5101/nbe.v3i1.p94-101

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Published: 30 June 2013
© 2013 S. Lan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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