AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Biomedicine and Engineering Article
PDF (2.6 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Article | Open Access

Activated Carbon Nanoparticles As Carriers of Anticancer Drugs

Lan Sun1, Yaoxin Cai2, Yanlei Liu1, Dongying Song3, Yan Liu1, Hongjuan Yao1, Yingge Zhang1( )
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing100850, China
Department of Pharmacy, Chinese PLA General Hospital, Beijing100853, China
Jilin University, Changchun 130021, China
Show Author Information

Abstract

The potentials of activated carbon nanoparticles (ACNP) as anticancer drug carriers were studied. ACNP were prepared with a top-down method. ACNP-based drug delivery system of docetaxel (ACNP-DOC) was prepared with a simple absorption method and its effects were studied primarily with methyl thiazolyl tetrazolium (MTT) assay, light microscope (LM), atomic force microscope (AFM) and transmission electron microscope (TEM). The prepared ACNP were approximately globular in shape with an average size of 233 nm, which had a saturate adsorption of 195.69 mg/g at mass ratio of ACNP: DOC=5:1. The drug delivery system prepared by adsorbing of ACNP for DOC had excellent releasing profile in the simulated in vivo environment. LM observation showed that ACNP can accumulate around the cells and on the surface of cells and TEM revealed that ACNP can enter cells and nuclears. MTT test demonstrated that ACNP-DOC had inhibitive effects on the growth of A549 cells, with an IC50 of 0.79 μg/ml, significantly smaller than that of 20 μg/ml of free DOC, indicating ACNP-DOC had stronger effects than free DOC. The imaging of AFM showed that both DOC and ACNP-DOC caused significant pathological changes of the cell membrane, including rough surfaces, large grains and holes, but these pathological changes were more obvious in ACNP-DOC treated cells than those free DOC treated ones. In all of the experiments, ACNP themselves had no significant influences on A549 cells. It was concluded that ACNP could serve as a good nanocarrier for anti-cancer drug delivery to target cells and have a great potential application in antitumor chemotherapy.

Keywords

Activated carbon nanoparticlesAtomic force microscopyA549 cellsDocetaxel

References

[1]

Zeng Z.B. The clinical app lication of actived carbon. Pract Clin Med. 2004. 5 (2):139-140.
Google Scholar
[2]

Hagiwara A., Takahashi T., Ueda T., Lee R., Takeda M., Itoh T. Intraoperative chemotherapy with carbon particles adsorbing mitomycin C for gastric cancer with peritoneal dissemination in rabbits. Surgery. 1988. 104(5):874-881.
Google Scholar
[3]

Qu Q.L., Zhang Y.G., Yang L.Z., Sun L. Intraperitoneal chemotherapy with mitomycin C bound to activated carbon nanoparticles for nude mice bearing human gastric carcinoma. Chin J Oncol. 2006. 28(4):257-260.
Google Scholar
[4]

Kam N.W., O’Connell M., Wisdom J.A., Dai H. Carbon nanotubes as multi-functional biological transporters and near-infrared agents for selective cancer cell destruction. Proc Nail Acad Sci USA. 2005. 102(33):11600-11605.
Crossref Google Scholar
[5]

Tongeren M.J., Kromhout H., Gardiner K. Trends in levels of inhalable dust exposure, exceedance and overexposure in the European carbon black manufacturing industry. Ann Occup Hyg. 2000. 44(4):271-280.
Crossref Google Scholar
[6]

Zhang Y.D. Nanobiotechnology. Beijing: Science Press. 2005. 1.
[7]

Yang Z., Zhao J.X., Li P.F., Zhang Y.G. Effects of activated carbon nanoparticles on anti cancer effect of 5-Fu. Bull Acad Mil Med Sci. 2009. 33(5):416-420.
Google Scholar
[8]

Zhang H., Sun L., Zhang Y.G. Intraperitoneal chemotherapy with mitomycin C bound to activated carbon nanoparticles using bioluminescence imaging technology. Mil Med Sci. 2011, 35(4): 299-302.
Google Scholar
[9]

Feazell R. P., Nakayama-Ratchford N., Dai H., Lippard S.J. Soluble single-walled carbon nanotubes as longboat delivery systems for platinum(Ⅳ) anticancer drug design. J. Am.Chem. Soc. 2007, 129 (27): 8438-8439.
Crossref Google Scholar
[10]

Zhu Y. H., Peng A. T., Carpenter K., Maguire J.A., Hosmane N.S., Takagaki M. Substituted carborane-appended water-soluble singlewall carbon nanotubes: new approach to boron neutron capture therapy drug delivery. J. Am. Chem. Soc. 2005, 127 (27):9875-9880.
Crossref Google Scholar
[11]

Zhang Z., Yang X., Zhang Y., Zeng B., Wang S., Zhu T., Roden R.B., Chen Y., Yang R. Delivery of telomerase reverse transcriptase small interfering RNA in complex with positively charged single-walled carbon nanotubes suppresses tumor growth. Clin. Cancer Res. 2006, 12(16):4933-4939.
Crossref Google Scholar
[12]

Kam N. W., O’Connell M., Wisdom J. A., Dai H. Proc. Natl. Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction. Acad. Sci. USA 2005, 102 (33):11600-11605.
Crossref Google Scholar
[13]

Pantarotto D., Briand J. P., Prato M., Bianco A. Translocation of bioactive peptides across cell membranes by carbon nanotubes. Chem. Commun (Camb). 2004, 10 (1):16-17.
Crossref Google Scholar
[14]

Kateb B., Van Handel M., Zhang L., Bronikowski M.J., Manohara H., Badie B. Internalization of MWCNTs by microglia: possible application in immunotherapy of brain tumors. NeuroImage 2007, 37(Suppl 1): S9-S17.
Crossref Google Scholar
[15]

Zhang Y.G. Nanotoxicology. Beijing: Peking union medical college press, 2010:256-265.
[16]

Huang G.J., Chen Z.G., Liu Y., Qian L.P., Chen J., Ni Q.X., Zhang Y.L. Guidance of activated carbon nanoparticles in gastric cancer lymphadenectomy. China Journal of Modern Medicin 2005, 15(2):233-237.
Google Scholar
[17]

Chen H., Huang G.J., Ni Q.X., Zhang Y.L. The application of nanometric activated carbon particles in the intralymphatic chemotherapy as a drug carrier. Fudan Univ. J. Med. Sci. 2007, 34(4):518-521.
Google Scholar
[18]

Stearns R.C., Paulauskis J.D., Godleski J.J. Endocytosis of Ul trafine particles by A549 cells. Am. J. Respir. Cell Mol. Biol. 2001, 24(2):108-115.
Crossref Google Scholar
[19]

Bitterle E., Karg E., Schroeppel A., Kreyling W.G., Tippe A., Ferron G.A., Schmid O., Heyder J., Maier K.L., Hofer T.Dosecontrolled exposure of A549 epithelial cells at the air-liquid interface to air borne ultrafine carbon aceous particles. Chemosphere. 2006, 65(10):1784-1790.
Crossref Google Scholar
[20]

Sayes C.M., Wahi R., Kurian P.A., Liu Y, West J.L., Ausman K.D., Warheit D.B., Colvin V.L. Correlating nanoscale titania structure with toxicity: A cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol. Sci. 2006, 92 (1):174-185.
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 5 Issue 2,
June 2013
Pages 94-101
DOI: 10.5101/nbe.v3i1.p94-101
Cite this article:
Sun L, Cai Y, Liu Y, et al. Activated Carbon Nanoparticles As Carriers of Anticancer Drugs. Nano Biomedicine and Engineering, 2013, 5(2): 94-101. https://doi.org/10.5101/nbe.v3i1.p94-101

390

Views

10

Downloads

3

Crossref

0

Scopus

Altmetrics
Published: 30 June 2013
© 2013 S. Lan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Return