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Article | Open Access

Gold Nanoparticles Enhance Efficiency of In Vitro Gene Transcription-Translation System

Daxiang Cui1,2( ), Hong Zhang1, Kan Wang2, Feng Gao2, Xueqing Zhang2, Toru Asahi1, Rong He2, Tetsuya Osaka1
Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, Japan
Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, People’s Republic of China
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Abstract

Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30% and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields’ bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.

Keywords

Gold NanoparticleRapid Translation SystemEfficiency

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Nano Biomedicine and Engineering
Volume 3 Issue 2,
June 2011
Pages 120-125
DOI: 10.5101/nbe.v3i2.p120-125
Cite this article:
Cui D, Zhang H, Wang K, et al. Gold Nanoparticles Enhance Efficiency of In Vitro Gene Transcription-Translation System. Nano Biomedicine and Engineering, 2011, 3(2): 120-125. https://doi.org/10.5101/nbe.v3i2.p120-125

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Published: 30 June 2011
© 2011 DX. C, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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