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Article | Open Access

Silver Nanoparticles: Novel Antimicrobial Agent Synthesized from an Endophytic Fungus Pestalotia sp. Isolated from Leaves of Syzygium cumini (L)

Farkanda RahemanShivaji DeshmukhAvinash IngleAniket GadeMahendra Rai()
Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, 444602, Maharashtra, India
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Abstract

We report the extracellular synthesis of silver nanoparticles using an endophytic fungus Pestalotia sp. isolated from leaves of Syzygium cumini (L) and their antibacterial activity against human pathogenic bacteria viz. Staphylococcus aureus (ATCC-25923) and Salmonella typhi (ATCC-51812) alone and in combination with commercially available antibiotics. Detection of synthesized silver nanoparticles was carried out using UV-Visible spectrophotometer analysis, which showed a peak at 415 nm indicating the formation of nanoparticles. Further characterization includes the Fourier Transform Infra-Red spectroscopic analysis for the detection of proteins as capping agents on nanoparticles. Nanoparticle Tracking and analysis (LM 20) and TEM analysis confirmed the formation of spherical and polydispersed nanoparticles in the range of 10-40 nm having average size of 12.40 nm.

Biologically synthesized silver nanoparticles showed significant antibacterial activity but their efficacy was increased in combination of antibiotics like gentamycin and sulphamethizole. Silver nanoparticles in combination with gentamycin showed maximum activity (30 mm) against S. aureus followed by sulphamethizole (25 mm). Similar results were reported in case of S. typhi where silver nanoparticles in combination with gentamycin (28 mm) showed more activity than combination of silver nanoparticles and sulphamethizole (24 mm).

Biosynthetic approach using an endophytic fungus is a novel way towards the development of safe, economically viable and green method for the synthesis of silver nanoparticles and thus synthesized silver nanoparticles can be used in antibacterial formulations.

References

1

Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am. J. Medicine. 2006; 119:3-10. http://dx.doi.org/10.1016/j.amjmed.2006.03.011

2

Mihail CR. Nanotechnology: convergence with modern biology and medicine. Curr. Opn. Biotechnol. 2003; 14:337-346. http://dx.doi.org/10.1016/S0958-1669(03)00068-5.

3

Birla SS, Tiwari VV, Gade AK, Ingle AP, Yadav AP, Rai MK. Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli. Pseudomonas aeruginosa and Staphylococcus aureus. Lett. in App. Microbio. 2009; 48(2):173-179 (2009). http://dx.doi.org/10.1111/j.1472-765X.2008.02510.x

4

Le AT, Huy PT, Tam LT, Tam PD, Hieu N, Huy T. Novel silver nanoparticles: synthesis, properties and applications. Int. J. of Nanotechnol. 2011; 8(3):278-290. http://dx.doi.org/10.1504/IJNT.2011.038205

5

Singh R, Singh NH, Medical Applications of Nanoparticles in Biological Imaging, Cell Labeling, Antimicrobial Agents, and Anticancer. Nanodrugs, J. Biomed. Nanotechnol. 2011; 7(4):489-503. http://dx.doi.org/10.1166/jbn.2011.1324

6

Gemmell CG, Edwards DI, Frainse AP. Guidelines for the prophylaxis and treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections in the UK. J. Antimicrob. Chemother. 2006; 57:589-608. http://dx.doi.org/10.1093/jac/dkl017

7

Chopra I, The increasing use of silver-based products as antimicrobial agents: a useful development or a cause for concern? J. Antimicrob. Chemother. 2007; 59:587-90. http://dx.doi.org/10.1093/jac/dkm203

8

Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Curr. Nano. 2008; 4:141-44. http://dx.doi.org/10.2174/157341308784340804

9

Ingle I, Gade A, Bawaskar M, Rai M. Fusarium solani: a novel biological agent for the extracellular synthesis of silver nanoparticles. J. Nanopart. Res. 2011; 11:2079-85. http://dx.doi.org/10.1007/s11051-008-9573-y

10

Gade AK, Bonde P, Ingle AP, Marcato PD, Duran N, Rai MK. Exploitation of Aspergillus niger for synthesis of silver nanoparticles, J. Biobased. Mater. Bioener. 2008; 2:243-247. http://dx.doi.org/10.1166/JPMB.2008.401

11

Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M. Fungusmediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine: Nanotech. Bio. and Med. 2009; 5:382-386. http://dx.doi.org/10.1016/j.nano.2009.06.005

12

Bawaskar M, Gaikwad S, Ingle A, Rathod D, Gade A, Duran N, Marcato PD, Rai M, A New Report on Mycosynthesis of Silver Nanoparticles by Fusarium culmorum, Curr. Nanosci. 2010; 6:376-380. http://dx.doi.org/10.2174/157341310791658919

13

Gade A, Ingle C, Whiteley, Rai M, Mycogenic metal nanoparticles: progress and applications. Biotechnol. Lett. 2010; 32(5):593-600. http://dx.doi.org/10.1007/s10529-009-0197-9

14

Gade A, Gaikwad S, Tiwari V, Yadav A, Ingle A, Rai M. Biofabrication of Silver Nanoparticles by Opuntia ficusindica: In vitro antibacterial activity and study of the mechanism involved in the synthesis, Curr. Nanosci. 2010; 6:370-75. http://dx.doi.org/10.2174/157341310791659026

15

Govindaraju K, Tamilselvan M, Kiruthiga V, Singaravelu G. Biogenic silver nanoparticles by Solanum torvum and their promising antimicrobial activity Antimicrobial activity of silver nanoparticles, Journal of Biopesticides. 2010; 3(1):394-399.

16

Namasivayam SKR, Ganesh S, Avimanyu. Evaluation of antibacterial activity of silver nanoparticles synthesized from Candida glabrata and Fusarium oxysporum. Int. J. Med. Res. 2011;1(3):131-136.

17

Cao G, Editor, Nanostructure and Nanomaterials: Synthesis, Properties and applications, Imperial college press, London 2004.

18

Duran N, Marcato PD, De Souza GI, Alves H, Esposito E. Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. J. Biomed. Nanotech. 2007; 3, 203-8. http://dx.doi.org/10.1166/jbn.2007.022

19

Li Y, Leung P, Song QW, Newton E. Antimicrobial effects of surgical masks coated with nanoparticles. J. Hosp. Infect. 2006; 62:58-63. http://dx.doi.org/10.1016/j.jhin.2005.04.015

20

Ki YN. In vitro antimicrobial effect of the tissue conditioner containing silver nanoparticles. J. Adv. Prosthodont. 2011; 3:20-24. http://dx.doi.org/10.4047/jap.2011.3.1.20

21

Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotech. Advances. 2009; 27:76-83. http://dx.doi.org/10.1016/j.biotechadv.2008.09.002

22

Gardea-Torresedey JL, Gomez E, Jose-Yacaman M, Parsons JG, Peralta-Videa JR, Tioani H. Alfalfa sprouts: A natural source for the synthesis of silver nanoparticles. Langmuir. 2003; 19:1357-61. http://dx.doi.org/10.1021/la020835i

23

Jones SA, Bowler PG, Walker M, Parsons D. Controlling wound bioburden with a novel silver-containing Hydrofiber dressing. Wound Repair Regen. 2004; 12:288-294. http://dx.doi.org/10.1111/j.1067-1927.2004.012304.x

24

Sastry M, Ahmad A, Khan MI. Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr. Sci. 2003; 85(2):162-170.

25

Gole A, Dash C, Ramakrishnan V, Sainker SR, Mandale AB, Rao M. Sastry M. Pepsin-gold colloid conjugates: preparation, characterization and enzymatic activity. Langmuir. 2001; 17(5):1674-79. http://dx.doi.org/10.1021/la001164w

26

Basavaraja S, Balaji SD, Lagashetty A, Rajasab AH. Venkataraman A. Extracellular biosynthesis of silver nanoparticle using the fungus Fusarium semitectum. Mate. Res. Bullatin. 2008; 45(5): 1164-70. (2008). http://dx.doi.org/10.1016/j.materresbull.2007.06.020.

27

Montes-Burgos I, Walczyk D, Hole P, Smith J, Lynch I. Dawson K. Characterisation of nanoparticle size and state prior to nanotoxicological studies J. Nanopart. Res. 2010; 12(1):47-53. doi10.1007/s11051-009-9774-z.

Nano Biomedicine and Engineering
Pages 174-178
Cite this article:
Raheman F, Deshmukh S, Ingle A, et al. Silver Nanoparticles: Novel Antimicrobial Agent Synthesized from an Endophytic Fungus Pestalotia sp. Isolated from Leaves of Syzygium cumini (L). Nano Biomedicine and Engineering, 2011, 3(3): 174-178. https://doi.org/10.5101/nbe.v3i3.p174-178
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