Abstract
Herein we reported the effects of generation 5 polyamidoamine dendrimer-functionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine embryonic stem cell CCE cells(ES) for 1 to 5 day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20 μg mL-1 dose caused ES cells became smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg mL-1 dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells’ differentiation at lower dose. The prepared dMNTs may be a good dual-phase regulation reagent to exposed ES cells, and have potential applications in research and development of ES cells.
