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Article | Open Access

Effects of Chiral Gold Nanoflowers on Polymerase Chain Reaction

Xiao Zhi, Xin Zhang, Peng Huang, Daxiang Cui( )
Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
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Abstract

In this paper, we reported that chiral gold nanoflowers with the concentration of 0.08 mM can enhance the specificity of polymerase chain reaction (PCR) and eliminate unspecific products, and the efficiency of PCR declines in some extent and the melt temperature of PCR products also raises slightly, which may be associated with the enhancement of the combining power of primers and ssDNA templates and the inner stability of dsDNA by chiral gold nanoflowers.The phenomena have great potential in applications such as ultrasensitive specific detection of DNA and SNPs.

Keywords

Gold NanoflowersChiralPCRReal-Time PCR

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Nano Biomedicine and Engineering
Volume 3 Issue 4,
December 2011
Pages 256-259
DOI: 10.5101/nbe.v3i4.p256-259
Cite this article:
Zhi X, Zhang X, Huang P, et al. Effects of Chiral Gold Nanoflowers on Polymerase Chain Reaction. Nano Biomedicine and Engineering, 2011, 3(4): 256-259. https://doi.org/10.5101/nbe.v3i4.p256-259

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Published: 31 December 2011
© 2011 X. Zhi.et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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