Abstract
In this paper, we reported that chiral gold nanoflowers with the concentration of 0.08 mM can enhance the specificity of polymerase chain reaction (PCR) and eliminate unspecific products, and the efficiency of PCR declines in some extent and the melt temperature of PCR products also raises slightly, which may be associated with the enhancement of the combining power of primers and ssDNA templates and the inner stability of dsDNA by chiral gold nanoflowers.The phenomena have great potential in applications such as ultrasensitive specific detection of DNA and SNPs.