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Article | Open Access

Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Flower Extract of Rhododendron dauricum

Amit Kumar Mittal, Abhishek Kaler, Uttam Chand Banerjee( )
Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar – 160062 Punjab, India
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Abstract

A simple and efficient eco-friendly approach for the biosynthesis of stable, monodisperse silver nanoparticles (AgNPs) using Rhododendron dauricum flower extract is described. Different reaction parameters (concentration of plant extract, substrate concentration, pH, temperature and reaction time) were optimized to synthesize AgNPs with controlled properties. AgNPs were characterized in terms of synthesis, size distribution (PDI of 0.25), capping functionalities (phenolic compounds) and microscopic evaluation by UV-Visible spectroscopy, dynamic light scattering, Fourier Transform Infrared (FTIR) spectroscopy and Transmission Electron Microscope (TEM). The specific characteristics and loss of organic content (1.81 mg) of the synthesized nanoparticles was measured by Differential Scanning Calorimetry (DSC) and Thermo Gravimetric Analysis (TGA). The results showed the simplistic and feasible approach for obtaining stable aqueous monodispersive AgNPs. Further, the antioxidant activity of AgNPs imparted by plant phenolic components was evaluated using DPPH assay and found to be comparable to standard TROLOX.

Keywords

Green synthesisSilver nanoparticlesPlant extractsAntioxidants assay

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Nano Biomedicine and Engineering
Volume 4 Issue 3,
September 2012
Pages 118-124
DOI: 10.5101/nbe.v4i3.p118-124
Cite this article:
Mittal AK, Kaler A, Banerjee UC. Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Flower Extract of Rhododendron dauricum. Nano Biomedicine and Engineering, 2012, 4(3): 118-124. https://doi.org/10.5101/nbe.v4i3.p118-124

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Published: 30 September 2012
© 2012 A.K. Mittal, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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