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Article | Open Access

Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ

Murugesan Suresh Kumar1, Anish Babu2, Ramachandran Murugesan2,3, Kadarkaraithangam Jeyasubramanian1( )
Department of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi-626005, INDIA
School of Biological Sciences, Madurai Kamaraj University, Madurai-625021, INDIA
Facuty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai-600310, INDIA
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Abstract

The present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic therapy (PDT) agent, protoporphyrin Ⅸ (PpⅨ). The (dark and photo) cytotoxicity of the PGSA-PpⅨ (dendrimer-drug) formulation towards Dalton Lymphoma Ascites (DLA) cancer cell lines upon visible light treatment is reported and evaluated the cytotoxic Reactive Oxygen Species (ROS) generation efficiency of the Protoporphyrin Ⅸ in free and dendrimer encapsulated forms. The in vitro toxicity demonstrated by PpⅨ loaded PGSA dendrimer nanoformulation, on DLA cells reveals that this novel PGSA nanocarrier reduces the toxic nature of PpⅨ when compared to free PpⅨ without light treatment which satisfy the aim of our study. On the other hand, treatment of DLA cells with PGSA dendrimer formulation in combination with light resulted in significant enhancement in therapeutic efficacy of the PDT agent, PpⅨ.

Keywords

Photodynamic TherapyDendrimerPhloroglucinol-Succinic acidnano carrierPpⅨROS generationin vitro toxicity

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Nano Biomedicine and Engineering
Volume 4 Issue 3,
September 2012
Pages 132-138
DOI: 10.5101/nbe.v4i3.p132-138
Cite this article:
Kumar MS, Babu A, Murugesan R, et al. Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ. Nano Biomedicine and Engineering, 2012, 4(3): 132-138. https://doi.org/10.5101/nbe.v4i3.p132-138

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Published: 30 September 2012
© 2012 M.S. Kumar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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