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Article | Open Access

Wound healing properties of modified silver nanoparticles and their distribution in mouse organs after topical application

Seda Keleştemur1, Ertugrul Kilic1,2, Ünal Uslu2, Alev Cumbul2, Milas Ugur1,2, Süleyman Akman3, Mustafa Culha1( )
Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Turkey
Faculty of Medicine, Yeditepe University, Istanbul, Turkey
Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Istanbul, Turkey
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Abstract

Citrate reduced colloidal silver nanoparticles (c-AgNPs) as synthesized and modified with oligonucleotides (Oligo-AgNPs) are comparatively evaluated for their wound healing properties on animal models. The healing progress was monitored daily during nine days by measuring the wound diameter. The tissue samples from the healed regions were analyzed for epithelial damage, congestion, inflammatory cell infiltration, fibroblast proliferation, and new collagen synthesis. The c-AgNPs and Oligo-AgNPs had statically significant impact on the healing process compared to control. The histological analysis revealed that the c-AgNPs and Oligo-AgNPs improved the congestion, inflammatory cell infiltration, fibroblast proliferation and new collagen synthesis as compared to control. Although the fibroblast proliferation seems to be the same for both c-AgNPs and Oligo-AgNPs, the collagen synthesis is markedly improved with the Oligo-AgNPs. The atomic spectroscopy analysis of the samples from different tissues showed that the AgNPs applied topically to the skin does not pass through the other organs. Our data suggest that topical application of Oligos-AgNPs improve wound healing by promoting increased collagen synthesis and tissue re-modeling without any side effects.

Keywords

congestionwound healingsilver nanoparticlescollagen synthesis inflammation

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Nano Biomedicine and Engineering
Volume 4 Issue 4,
December 2012
Pages 170-176
DOI: 10.5101/nbe.v4i4.p170-176
Cite this article:
Keleştemur S, Kilic E, Uslu Ü, et al. Wound healing properties of modified silver nanoparticles and their distribution in mouse organs after topical application. Nano Biomedicine and Engineering, 2012, 4(4): 170-176. https://doi.org/10.5101/nbe.v4i4.p170-176

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Published: 31 December 2012
© 2012 S. Keleştemur et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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