AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Biomedicine and Engineering Article
PDF (707.6 KB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Article | Open Access

Biogenic Synthesis of Silver Nanoparticles Using Saraca indica Leaf Extract and Evaluation of Their Antibacterial Activity

Ravi Mani Tripathia,b,c, Deepti Ranac, Archana Shrivastava( ), Singh Rajendra Palc, Braj Raj Shrivastavd,e
Department of Microbiology, College of Life Sciences, Gwalior - 474 009 (M.P.), India
School of Science in Biotechnology, Jiwaji University, Gwalior - 474 011 (M.P.), India
Amity Institute of Nanotechnology, Amity University, Sector- 125, Noida- 201303, India
Department of Surgical Oncology, Cancer Hospital & Research Institute, Gwalior - 474 009 (M.P.), India
Department of Surgery, G. R. Medical College, Palace Road, Gwalior - 474009 (M.P.), India
Show Author Information

Abstract

The present study reports an unprecedented biogenic method for the synthesis of silver nanoparticles (AgNPs) using leaf extract of Saraca indica and characterized their antibacterial activity. We have also focused on the biosynthesis mechanism of AgNPs. Plant leaf extract has water soluble organic materials which help in the reduction of silver ions and stabilization of AgNPs. Aqueous solution of silver nitrate was treated with leaf extract of Saraca indica for the formation of AgNPs. The surface plasmon resonance was occurred at 412 nm. The size distribution profile of synthesized AgNPs was analysed by Dynamic Light Scattering (DLS). Transmission Electron Microscopy (TEM) has been done for the measurement of particle size and their morphology. The role of phytochemicals in the reduction of silver ions and defining the framework in which AgNPs are covered and provide steadiness can be determined through Fourier Transform Infrared Spectroscopy (FTIR). TEM micrograph reveals that the size of AgNPs was obtained in the range of 13-50 nm with spherical morphology. X-ray Diffraction (XRD) pattern of the AgNPs exhibited 2θ values corresponding to the silver nanocrystals. Furthermore, the antibacterial activity of synthesized AgNPs against E. coli DH5α was investigated by growth curve and inhibition zone analysis. It was observed that the 20 μg/ml concentration of biogenic AgNPs recorded as minimal inhibitory concentration (MIC) against E. coli DH5α.

Keywords

Antibacterial activitySilver nanoparticlesSaraca indicaE. coli DH5αWell diffusion method

References

[1]

Saxena A., Tripathi R.M., Zafar F., Singh P., Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Mater Lett. 2012; 67: 91-94.
Crossref Google Scholar
[2]

Kumar V., Yadav C.S., Yadav K.S., Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterisation. J. Chem. Sci. 2010; 85: 1301-1309.
Crossref Google Scholar
[3]

Gittins D.I., Bethell D., Schiffrin D.J., Nichols R.J., A nanometre scale electronic switch consisting of a metal cluster and redox- addressable groups. Nature 2000; 408: 67-69.
Crossref Google Scholar
[4]

Mandal D., Bolander M.E., Mukhopadhyay D., Sarkar G., Mukherjee P., The use of microorganisms for the formation of metal nanoparticle and their applications. Appl. Microbiol. 2006; 69: 485-492.
Crossref Google Scholar
[5]

Raveendran P., Fu J., and Wallen S L. Completely “green”synthesis and stabilization of metals nanoparticles. J. Am. Chem. Soc.2003; 125: 13940-13941.
Crossref Google Scholar
[6]

Ji M., Chen X., Wai C.M., Fulton J.L., Synthesizing and dispersing silver nanoparticles in a water-in-supercritical carbon dioxide micro- emulsion. J. Am. Chem. Soc. 1999; 121: 2631-2632.
Crossref Google Scholar
[7]

Klaus T., Joerger R., Olsson E., Granquist C.G., Silver- based crystalline nanoparticles, microbially fabricated. Proc. Natt. Acad. Sci. USA. 1999; 96: 13611-13614.
Crossref Google Scholar
[8]

Nair B., Pradeep T., Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains. Cryst. Growth. Des. 2002; 2: 293-298.
Crossref Google Scholar
[9]

Panacek A., Kvitek L., Prucek R., Kolar M., Veerova R., Pizurova N., Sharma V.K., Nevena T., Zboril R., Silver colloid nanoparticles: synthesis, characterisation and their antibacterial activity. J. Phys. Chem. B. 2007; 110: 16248-16253.
Google Scholar
[10]

Jung W.K., Koo H.C., Kim K.W., Shin S., Kim S.H., Park Y.H., Antibacterial activity and mechanism of action of the silver ions in Staphylococcus aureus and E. coli. Appl. Environ. Microb. 2008; 74: 2171-2178.
Crossref Google Scholar
[11]

Lee H.Y., Park H.K., Lee Y.M., Kim K., Park S.B., A practical procedure for producing silver nano film coated fabric and its antibacterial evaluation for biomedical applications. Chem. Commun. 2007; 28: 2959-2967.
Crossref Google Scholar
[12]

Kumar V., Yadav S.K., Plant-mediated synthesis of silver and gold nanoparticle and their applications. J. Chem. Technol. Biot. 2009; 84: 151-157.
Crossref Google Scholar
[13]

Mohanpuria P, Rana N.K., Yadav S.K., Biosynthesis of nanoparticles: technological concepts and future applications. J. Nanopart. Res. 2008;10: 507-517.
Crossref Google Scholar
[14]

Mukherjee P., Roy M., Mandal B.P., Dey G., Mukherjee K., GhatakP.K., Tyagi J., Kale A.K.S.P., Green synthesis of highly stabilised nanocrystalline silver particles by a non-pathogenic and agricultural important fungus T asperellum. Nanotechnology. 2008; 19: 788-800.
Crossref Google Scholar
[15]

Song J.K., Kim B.S., Rapid biological synthesis of silver nanoparticle using plant leaf extracts. Bioproc. Biosyst. Eng. 2009; 32: 79-82.
Crossref Google Scholar
[16]

Shankar S.S., Ahmad A., Sastry M., Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol. Progr . 2003; 19: 1627-1631.
Crossref Google Scholar
[17]

Li S., Shen Y., Xie A., Yu X., Qui L., Zhang L., Zhang Q., Green synthesis of silver nanoparticles using Capsicum annuum extract. Green. Chem. 2007; 9: 852-858.
Crossref Google Scholar
[18]

Shankar S.S., Rai A., Ahmad A., Sastry M., Rapid synthesis of Au, Ag and bimetallic Au core- Ag shell of nanoparticles using Neem (Azadirachta indica) leaf broth. Interface Sci. 2004; 275: 496-502.
Crossref Google Scholar
[19]

Saxena Antariksh, Tripathi R.M., Singh R.P., Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Dig. J. Nanomater. Bios. 2010; 5: 427-432.
Google Scholar
[20]

Nadagouda M.N., Varma R.S., Green synthesis of silver and palladium nanoparticles at room temperature using coffee and tea extract. Green Chem. 2008; 10: 859-862.
Crossref Google Scholar
[21]

Gardea-Torresdey J.L., Gomez E., Peralta-Videa J.R., Parsons J.G., Troiani H., Jose-Yacaman M., Alfalfa sprouts of natural source for the synthesis of silver nanoparticles. Langmuir. 2003; 19: 1357-1361.
Crossref Google Scholar
[22]

Haverkamp R.G., Marshall A.T., The mechanism of metal nanoparticle formation in plants: limits on accumulation. J. Nanopart. Res. 2009; 11: 1453-1463.
Crossref Google Scholar
[23]

Huang J., Li Q., Sun D., Lu Y., Su Y., Yang X., Wang H., Wang Y., Shao W., Hong N.J., Chen C., Biosynthesis of silver and gold nanoparticles by novel Sundried Cinnamomum camphora Leaf, ” Nanotechnology. 2007; 18: 105104-105115.
Crossref Google Scholar
[24]

Wei H., Chen C., Han B., Wang E., Enzyme colorimetric assay using unmodified silver nanoparticles. Anal. Chem. 2008; 80: 7051-7055.
Crossref Google Scholar
[25]

Jain P., Pradeep T., Potential of silver nanoparticle-coatedpolyurethane foam as an antibacterial water filter. Biotechnol. Bioeng. 2005; 90: 59-63.
Crossref Google Scholar
[26]

Shiraishi Y., Toshim N., Oxidation of ethylene catalysed by colloidal dispersions of poly (Sodium acrylate)-protected silver nanoclusters. Colloid Surface A. 2000; 169: 59-66.
Crossref Google Scholar
[27]

Elechiguerra J.L., Burt J.L., Morones J.R., Camacho-Bragado A., Gao X., Lara H.H., Interaction of nanoparticles with HIV-1. J. Nanobiotechnol. 2005; 3: 6.
Crossref Google Scholar
[28]

Verma A., Jana K.G., Sen S., Chakraborty R., Sachan S., Mishra A., Pharmacological evaluation of Saraca indica leaves for central nervous system depressant activity in mice. J. Pharm. Sci. and Res. 2010; 2: 338-343.
Google Scholar
[29]

Okamura H., Mimura A., Niwan M., Yakou Y., Takahara Y., Antioxidant activity of tannins and flavonoids in Eucalyptus rostrata. The International J. Plant Biochem. 1993; 33: 557-561.
Crossref Google Scholar
[30]

Van Acker S.A., Van den berg D.J., Griffioen D.H., Van Bennekom W.P., Van der Vijgh W.J.F., Tormp M.N., Structural aspects of antioxidant activity of Flavonoids. Free Radical Bio. Med. 1996; 20: 331-342.
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 5 Issue 1,
March 2013
Pages 50-56
DOI: 10.5101/nbe.v5i1.p50-56
Cite this article:
Tripathi RM, Rana D, Shrivastav A, et al. Biogenic Synthesis of Silver Nanoparticles Using Saraca indica Leaf Extract and Evaluation of Their Antibacterial Activity. Nano Biomedicine and Engineering, 2013, 5(1): 50-56. https://doi.org/10.5101/nbe.v5i1.p50-56

283

Views

12

Downloads

20

Crossref

0

Scopus

Altmetrics
Published: 30 March 2013
© 2013 R.M. Tripathi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Return