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Article | Open Access

Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract

Singaravelu Senthamilselvi1( ), Ponnuchamy Kumar2, Alagiyamanavalan Lakshmi Prabha1, Munisamy Govindaraju2
Department of Plant Science, School of Life Sciences. Bharathidasan University, Tiruchirappalli – 620 024
Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli – 620 024
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Abstract

Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly within 20 min. High throughput characterization such as UV-vis spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Field Emission-Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Zeta potential measurements disclose the existence of silver nanoparticles. The phenolic compounds present in the aqueous leaves extract paves way for the possible reduction of silver to nano-silver. Electron microscopic studies revealed the average size of nanoparticles were 52 nm. The nanoparticles are highly stable by reaching a zeta potential value of −33.6 mV. Comparative antibacterial efficacy of silver nanoparticles was investigated by disk diffusion and microtitre broth dilution method against Escherichia coli. The result showed that silver nanoparticles are toxic to E. coli cells at higher concentration. Overall, we suggest microtitre broth dilution method is more reliable to determine the antimicrobial activity of silver nanoparticles than disk diffusion method.

Keywords

E. coliGreen nanotechnologyAnnona squamosadisk diffusionmicrotitre broth dilution method

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Nano Biomedicine and Engineering
Volume 5 Issue 2,
June 2013
Pages 102-106
DOI: 10.5101/nbe.v5i2.p102-106
Cite this article:
Senthamilselvi S, Kumar P, Prabha AL, et al. Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract. Nano Biomedicine and Engineering, 2013, 5(2): 102-106. https://doi.org/10.5101/nbe.v5i2.p102-106

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Published: 30 June 2013
© 2013 S. Senthamilselvi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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