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Article | Open Access

Fluoroimmunoassay for Antigen Based on Fluorescence Resonance Energy Transfer Between Quantum Dots and Gold Nanoparticles

Can Wang1, Xiaogang You2, Hualin Fu2, Peng Huang1, Feng Gao2, Yunsheng Chen2, QuanDe Liao1( )
Xiangya Hospital of Central South University, 87 Xiangya Road, Changsha410008, Hunan, P. R. China
Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders of Ministry of Education, Bio-X Center, Shanghai JiaoTong University, Dongchuan Road 800, 200240 Shanghai, P. R. China
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Abstract

A unique, sensitive, and highly specific fluoroimmunoassay system for antigen detection using quantum dot and gold nanoparticles has been developed. The assay is based on the fluorescence quenching of quantum dots caused by gold nanoparticles coated with antibody. To demonstrate its analytical capabilities, the quantum dots were coated with anti-HBsAg monoclonal antibodies (QDs-MAb1) and gold nanoparticles coated with another anti-HBsAg monoclonal antibodies (GNPs-MAb2) which specifically bound with HBsAg could sandwich the HBsAg captured by the immunoreactions. The sandwich-type immunocomplex was formed and the energy of quantum dots was transferred to gold nanoparticles as they were within a short distance, so that the fluorescence intensity of quantum dots was quenched. The fluorescence intensity of quantum dots at 570 nm was negative linear proportional to HBsAg concentration logarithm. The result showed that the limit of detection of the HBsAg was 0.928 ng/mL. This new system can be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and disease diagnosis.

Keywords

Quantum DotsGold NanoparticlesAntigenFluorescence resonance energy transferFluoroimmunoassay

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Nano Biomedicine and Engineering
Volume 5 Issue 3,
September 2013
Pages 127-130
DOI: 10.5101/nbe.v5i3.p127-130
Cite this article:
Wang C, You X, Fu H, et al. Fluoroimmunoassay for Antigen Based on Fluorescence Resonance Energy Transfer Between Quantum Dots and Gold Nanoparticles. Nano Biomedicine and Engineering, 2013, 5(3): 127-130. https://doi.org/10.5101/nbe.v5i3.p127-130

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Published: 30 September 2013
© 2013 C. Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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