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Article | Open Access

Dendrimer modified SWCNTs for High Efficient Delivery and Intracellular Imaging of survivin siRNA

Can Wang1, Zhiming Li2, Bing Liu2, Quande Liao1( ), Chenchen Bao2, Hualin Fu2, Bifeng Pan2, Weilin Jin2, Daxiang Cui2( )
Xiangya Hospital of Central South University, 87 Xiangya Road, Changsha410008, Hunan, P. R. China
Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders of Ministry of Education, Bio-X Center, Shanghai JiaoTong University, Dongchuan Road 800, 200240 Shanghai, P. R. China
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Abstract

Herein we reported that polyamidoamine dendrimer (PAMAM) modified single walled carbon nanotubes (PSWCNTs) were successfully used for high efficient delivery and intracellular imaging of survivin siRNA vector. No.4 generation of PAMAM dendrimers were used to modify single walled carbon nanotubes, and characterized by high resolution transmission electron microscopy (HR-TEM), and atom force microscopy (AFM), survivin shRNA vectors were constructed and identified, and then dendrimer-modified SWCNTs were mixed with survivin shRNA vectors, resultant dendrimer-SWCNTs-survivin shRNA vector mixtures co-cultured with human gastric cancer MGC803 cells, then the expression of survivin shRNA vectors in MGC803 cells was observed by fluorescent microscopy, cell viability was analyzed by MTT method. Results showed that survivin shRNA vector was successfully expressed in MGC803 cells, and MGC 803 cell growth was markedly inhibited. In conclusion, dendrimer (PAMAM) modified single walled carbon nanotubes can be used for high efficient delivery and intracellular imaging of siRNA, and own great potential in applications such as gene or drug delivery and tumor targeted imaging and simultaneous therapy in near future.

Keywords

ImagingsiRNADelivery systemPolyamidoamine dendrimerSingle walled carbon nanotube

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Nano Biomedicine and Engineering
Volume 5 Issue 3,
September 2013
Pages 131-136
DOI: 10.5101/nbe.v5i3.p131-136
Cite this article:
Wang C, Li Z, Liu B, et al. Dendrimer modified SWCNTs for High Efficient Delivery and Intracellular Imaging of survivin siRNA. Nano Biomedicine and Engineering, 2013, 5(3): 131-136. https://doi.org/10.5101/nbe.v5i3.p131-136

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Published: 30 September 2013
© 2013 C. Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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