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Research Article | Open Access

Biosynthesis of Silver Nanoparticles Using Two Seaweeds and Their Potential Towards Environment

Dhanalakshmi Aravindan, Riyazulla Azeez, Thangaraju Nallamuthu( )
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Guindy, Chennai 600025
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Abstract

Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine. Biologically synthesized silver nanoparticles have a wide range of applications. The biosynthesis of silver nanoparticles is an eco-friendly method in the field of nanotechnology. Seaweed extracts of Caulerpa racemosa (Forsskål) J. Agardh and Ulva lactuca Linnaeus was used as a reducing agent in the eco-friendly extracellular synthesis of silver nanoparticles from an aqueous solution of silver nitrate (AgNO3). High conversion of silver ions to silver nanoparticles was achieved with Ulva lactuca at reaction temperature of 100 ℃ and a seaweed extract concentration of 10% with a residential time of 1 h using reflux extractor when compared with the other methods. Formation of silver nanoparticles was characterized by spectrophotometry and the electron microscopic technique. The average particles size was ranging from 35 to 75 nm. Antimicrobial activities indicate the minimum inhibitory concentration of biologically synthesized nanoparticles tested against the pathogen Staphylococcus aureus (1 mg/ml). High inhibitions over the growth of Pseudomonas aeruginosa, Vibrio cholerae and Escherichia coli were witnessed against the concentrations of 1 mg/ml. Enzyme assay of the collected seaweeds performed using standard protocol to assess the potency level. Further, seed germination test proved that synthesized nanoparticles were environmentally safe, for which the same can be used for effluent treatment process.

Keywords

Photocatalytic degradationBionanoparticlesAntimicrobial activitySeed germination

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Nano Biomedicine and Engineering
Volume 6 Issue 4,
December 2014
Pages 94-103
DOI: 10.5101/nbe.v6i4.p94-103
Cite this article:
Aravindan D, Azeez R, Nallamuthu T. Biosynthesis of Silver Nanoparticles Using Two Seaweeds and Their Potential Towards Environment. Nano Biomedicine and Engineering, 2014, 6(4): 94-103. https://doi.org/10.5101/nbe.v6i4.p94-103

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Received: 22 September 2014
Accepted: 15 December 2014
Published: 25 December 2014
© 2014 Dhanalakshmi Aravindan, Riyazulla Azeez and Thangaraju Nallamuthu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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