AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
PDF (24.3 MB)
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

Leaf Assisted Green Synthesis of Silver Nanoparticles from Syzygium Alternifolium (Wt.) Walp. Characterization and Antimicrobial Studies

Pulicherla Yugandhar( )Nataru Savithramma
Dept. of Botany, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India
Show Author Information

Abstract

Development of environmentally benign methods for synthesis of nanoparticles is an evolving important branch of nanotechnology. In the present study we report a green method for synthesis of stable silver nanoparticles (SNPs) from aqueous leaf extract of Syzygium alternifolium an endemic medicinal plant to Southern Eastern Ghats. These green synthesized nanoparticles are characterized by using UV-VIS spectroscopy, FTIR, XRD, AFM, SEM with EDAX and TEM. The colour change from yellow to grey is observed upon synthesis and 448 nm peak was obtained from UV-VIS spectroscopic analysis. FTIR spectroscopic studies confirms that phenols and proteins of leaf extract is main responsible for capping and stabilization of these SNPs. Crystallographic studies from XRD indicated the SNPs are crystalline in nature and owing 44 nm size. High resolution and magnification studies with AFM, SEM and TEM analysis revealed that the nanoparticles are spherical in shape having the size range from 7 to 44 nm. EDAX pattern of synthesized SNPs showed 47.28 weight percentage of Ag metal in the sample indicate the purity of sample. Further, the antimicrobial studies of these green synthesized SNPs show high toxicity towards different bacterial and fungal isolates. The results revealed that the selected medicinal plant possess the potentiality towards the synthesis of narrow range nanoparticles also combat with the pathogens.

References

[1]

S. Maensiria, P. Laokula, J. Klinkaewnaronga, et al., Indium oxide (In2O3) nanoparticles using Aloe vera plant extract: Synthesis and optical properties. J. Optoelectron. Adv. Mater. , 2008, 10: 161-165.

[2]

R. Herrera-Becerra, C. Zorrilla, J.L. Rius, J.A. Ascencio. Electron microscopy characterization of biosynthesized iron oxide nanoparticles. Appl. Phys. A: Mater. Sci. Process, 2008, 9: 241-246.

[3]

X. Yang, L. Qingbiao, H. Wang, et al., Green synthesis of palladium nanoparticles using broth of Cinnamomum camphora leaf. J. Nanopart. Res. , 2009, 12: 1589-1598.

[4]

H.J. Lee, J.Y. Song, B.S. Kim. Biological synthesis of copper nanoparticles using Magnolia kobus leaf extract and their antibacterial activity. J. Chem. Technol. Biotechnol, 2013, 88: 1971-1977.

[5]

K.A. Javad, M. Sasan. Phytosynthesis of Cadmium Oxide Nanoparticles from Achillea wilhelmsii Flowers. J. Chem. , 2013, 2013: 1-4.

[6]

P. Yugandhar, N. Savithramma. Green synthesis of calcium carbonate nanoparticles and their effects on seed germination and seedling growth of Vigna mungo (L.). Hepper. Int. J. Adv. Res. , 2013, 1: 89-103.

[7]

A. Veronica, H. Isaac, R.P.V. Jose, et al., Size controlled gold nanoparticle formation by Avenasativa biomass: use of plants in nanobiotechnology. J. Nanopart. Res. , 2004, 6: 377-382.

[8]

G. Bhumi, N. Savithramma. Biological Synthesis of Zinc oxide Nanoparticles from Catharanthus roseus (L.) G. Don. Leaf extract and validation for antibacterial activity. Int. J. Drug. Dev. Res. , 2014, 6: 208-214.

[9]

Ch. Marutikesava Kumar, P. Yugandhar, D. Suhrulatha, N. Savithramma. Synthesis, Characterization and Antimicrobial studies of stem bark mediated synthesis of silver nanoparticles from Adansonia digitata (L.). J. Pharm. Sci. Res. , 2015, 7: 76-82.

[10]

J.R. Morones, J.L. Elechiguerra, A. Camacho, et al., The bactericidal effect of silver nanoparticles. Nanotechnology, 2005, 16: 2346-2353.

[11]

N. Savithramma, M. Linga Rao, P. Suvarnalathadevi. Evaluation of antibacterial efficacy of biologically synthesized silver nanoparticles using stem barks of Boswellia ovalifoliolata Bal. and Henry and Shorea tumbuggaia Roxb. Journal of Biological Sciences, 2011, 11: 39-45.

[12]

N. Savithramma, M. Lingarao, S.K.M. Basha. Antifungal efficacy of silver nanoparticles synthesized from the medicinal plants. Der Pharma Chemica, 2011, 3: 364-372.

[13]

G. Seema, C. Amrish. Bio synthesis and anthelmintic activity of silver nanoparticles using aqueous extract of Saraca indica leaves. International Journal of Therapeutic Applications, 2012, 7: 9-12.

[14]

C. Sundaravadivelan, P.M. Nalini, P. Sivaprasanth, L. Kishmu. Biosynthesized silver nanoparticles from Pedilanthu stithymaloides leaf extract with anti-developmental activity against larval instars of Aedes aegypti L. (Diptera, Culicidae). Parasitol. Res. , 2013, 112: 303-311.

[15]

M. Kumara Swamy, K.M. Sudipta, K. Jayanta, S. Balasubramanya. The green synthesis, characterization, and evaluation of the biological activities of silver nanoparticles synthesized from Leptadenia reticulata leaf extract. Appl. Nanosci. , 2014, 5: 73-81.

[16]

K. Vasanth, K. Ilango, R. Mohan Kumar, et al., Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction. Colloids. Surf. B. , 2014, 1: 354-359.

[17]

H.M.E. Rafie, M.A.A. Hamed. Antioxidant and anti-inflammatory activities of silver nanoparticles biosynthesized from aqueous leaves extracts of four Terminalia species. Adv. Nat. Sci: Nanosci. Nanotechnol, 2014, 5: 1-11.

[18]

R. Bhuvaneswari, N. Chidambaranathan, K. Jegatheesan. Hepatoprotective effect of Embilica officinalis and its silver nanoparticles against ccl4 induced hepatotoxicity in wistar albino rats. Digest Journal of Nanomaterials and Biostructures, 2014, 9: 223-235.

[19]

G. Seema, C. Amrish, M. Avijit, M. Rupa. Green synthesis of silver nanoparticles using Arnebia nobilis root extract and wound healing potential of its hydrogel. Asian. J. Pharm. , 2014, 8: 95-101.

[20]

V. Yakolev, Y.O. Golubeva. Synthesis optimisation of Lysozyme Monolayer-Coated silver nanoparticles in aqueous solution. J. Nanomater, 2014, 2014: 1-8.

[21]

G.B. Neus, F. Merkoci, J. Piella, V. Puntes. Synthesis of Highly Monodisperse Citrate-Stabilized Silver Nanoparticles of up to 200 nm: Kinetic Control and Catalytic Properties. Chem. Mater. , 2014, 26: 2836-2846.

[22]

R. Zamiri, B.Z. Azmi, H.A. Ahangar, et al., Preparation and characterization of silver nanoparticles in natural polymers using laser ablation. Bull. Mater. Sci. , 2012, 35: 727-731.

[23]

J. Harra, J. Makitalo, R. Siikanen, et al., Size-controlled aerosol synthesis of silver nanoparticles for plasmonic materials. J. Nanopart. Res. , 2012, 14: 1-10.

[24]

S. Iravani, H. Korbekandi, S.V. Mirmohammadi, B. Zolfaghari. Synthesis of silver nanoparticles: chemical, physical and biological methods. Res. Pharm. Sci. , 2014, 9: 385-406.

[25]

K. Vithiya, R. Kumar, S. Sen. Bacillus sp. mediated extracellular synthesis of silver nanoparticles. Int. J. Pharm. Pharm. Sci. , 2014, 6: 525-527.

[26]

M. Anamika, C. Sanjukta, M.R. Prashant, W. Geeta. Evidence based green synthesis of nanoparticles. Adv. Mater. Lett. , 2012, 3: 519-525.

[27]

K.N. Reddy, C.S. Reddy. First Red List of medicinal plants of Andhra Pradesh, India-Conservation assessment and management planning. Ethnobotanical Leaflets, 2008, 12: 103-107.

[28]

N. Savithramma, P. Yugandhar, R. HariBabu, K. Siva Prasad. Validation of Indigenous Knowledge of Yanadi Tribe and Local Villagers of Veyilingalakona- A Sacred Grove of Andhra Pradesh, India. J. Pharm. Sci. Res. , 2014, 6: 382-388.

[29]

N. Savithramma, P. Yugandhar, M. Lingarao. Ethnobotanical Studies on Japali Hanuman Theertham- A Sacred Grove of Tirumala hills, Andhra Pradesh, India, J. Pharm. Sci. Res. , 2014, 6: 83-88.

[30]

S. Karuppusamy, G. Muthuraja, K.M. Rajasekaran. Lesser Known Ethnomedicinal Plants of Alagar Hills, Madurai District of Tamil Nadu, India, Ethnobotanical Leaflets, 2009, 13: 1426-1433.

[31]

A. Sudhakar, C. Ramesh, N. Nagaraju, et al., Pharmacognostical Studies on Stem & Fruit of Syzygium alternifolium (Wight) Walp- An Endemic to South Eastern Ghats, India. Asian J. Biochem. Pharm. Res. , 2012, 1: 127-138.

[32]

V.V.S.S. AppalaRaju, M. Ramesh, M. Lakshmi Narsau, M. Muralikrishna Kumar. Antimicrobial Activity of the Plant Syzygium alternifolium. Asian. J. Chem. , 2007, 19: 4923-4924.

[33]

A. Rama Mohan, K.V.S.R.G. Prasad, J.A.R.P. Sharma, Hypoglycemic and antihyperglycemic activity of Syzygium alternifolium (Wt.) Walp. leaf extracts in normal and diabetic rats. Int. J. Drug Dev. Res. , 2010, 2: 27-32.

[34]

R.K. Sreelatha Devi, P. Sreenivasulu, S.K.M. Basha. Antioxidant activity and total polyphenols content of certain high valued medicinal plants of tirumala hills, Andhra Pradesh. Indian J. Plant Sci. , 2013, 2: 93-98.

[35]

B. Komuraiah, C. Srinivas, A. Niranjana Kumar, et al., Isolation of Phytochemicals From Anticancer Active Extracts of Syzygium alternifolium Walp. Leaf. Phcog. J. , 2014, 6: 83-85.

[36]

R. Cruickshank. Medical microbiology: a guide to diagnosis and control of infection. Livingston publishers, Edinburghand London, 1986.

[37]

R.M. Gengana, K. Ananda, A. Phulukdaree, et al., A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf. Colloids and Surfaces B: Biointerfaces, 2013, 105: 87-91.

[38]

G. Sharma, A.R. Sharma, M. Kurian, et al., Green synthesis of silver nanoparticle using Myristica fragrans (nutmeg) seed extract and its biological activity. Digest Journal of Nanomaterials and Biostructures, 2014, 9: 325-332.

[39]

N. Jain, A. Bhargava, S. Majumdar, et al., Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: A mechanism perspective. Nanoscale, 2011, 3: 635-641.

[40]

P. Yugandhar, N. Savithramma, Biosynthesis, characterization and antimicrobial studies of green synthesized silver nanoparticles from fruit extract of Syzygium alternifolium (Wt.) Walp. an endemic, endangered medicinal tree taxon. Appl Nanosci, 2015, DOI: 10.1007/s13204-015-0428-4.

[41]

M.M.H. Khalil, E.H. Ismail, K.Z.E. Baghdady, et al., Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian J. Chem. , 2014, 7: 1131-1139.

[42]

E.K. Elumalai, T.N.V.K.V. Prasad, V. Kambala, et al., Green synthesis of silver nanoparticle using Euphorbia hirta L and their antifungal activities. Arch. Appl. Sci. Res. , 2010, 2: 76-81.

[43]

I. Sondi, B.S. Sondi, Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gramnegative bacteria. J. Colloid. Interface. Sci. , 2004, 275: 177-182.

[44]

C. Krishnaraj, R. Ramachandran, K. Mohan, et al., Optimization for rapid synthesis of silver nanoparticles and its effect on phytopathogenic fungi. Spectrochim Acta, Part A, 2012, 93: 95-99.

[45]

C. Baker, A. Pradhan, L. Pakstis, et al., Synthesis and antibacterial properties of silver nanoparticles. J. Nanosci. Nanotechnol, 2005, 5: 24-29.

Nano Biomedicine and Engineering
Pages 29-37
Cite this article:
Yugandhar P, Savithramma N. Leaf Assisted Green Synthesis of Silver Nanoparticles from Syzygium Alternifolium (Wt.) Walp. Characterization and Antimicrobial Studies. Nano Biomedicine and Engineering, 2015, 7(2): 29-37. https://doi.org/10.5101/nbe.v7i2.p29-37

335

Views

10

Downloads

10

Crossref

16

Scopus

Altmetrics

Received: 10 February 2015
Accepted: 24 April 2015
Published: 30 April 2015
© 2015 Pulicherla Yugandhar and Nataru Savithramma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Return