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Research Article | Open Access

Evaluation of Antibacterial Activity and Cytotoxicity of Green Synthesized Silver Nanoparticles Using Scoparia Dulcis

Kalyani Khanra1, Sudipta Panja1, Indranil Choudhuri1, Anindita Chakraborty2, Nandan Bhattacharyya1( )
Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India
Radiation Biology Division,UGC-DAE CSR, Kolkata Centre, Sector Ⅲ, LB-8, Bidhan Nagar, Kolkata 700098, India
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Abstract

Green synthesis of silver nanoparticles (AgNPs) is gaining momentum in the field of nano-research. Scoparia dulcis leaves were used as a reducing agent for the synthesis of silver nanoparticles from an aqueous solution of silver nitrate (AgNO3). Synthesized AgNPs were characterized by UV-Vis spectroscopy, XRD, SEM with EDAX and TEM. UV-VIS surface plasmon resonance spectroscopy was observed at 430 nm. XRD data depicts that the NPs are crystalline in nature. The EDAX data indicate that 63.76% presence of Ag metal. The TEM & SEM results indicate that size of the AgNPs ranges from 15-25 nm. The results also support that spherical shape of the nanoparticles. In addition, the NPs are in polydispersed condition. The antimicrobial activities indicate significant inhibition of the growth of three pathogenic bacteria such as Pseudomonas ariginosa, Bacillua subtillis and Staphylococcus aureous. Cytotoxicity of this nanoparticle showed that this Ag-NP also has more cytotoxic effect on a lung cancer cell line, A549 cells compared to ovarian cancer cell line, PA1 indicating a possible therapeutic use of this AgNP.

Keywords

Silver nanoparticlesAntimicrobial activityPlant extractGreen Silver nanoperticle synthesiscytotoxicity assay

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Nano Biomedicine and Engineering
Volume 7 Issue 3,
September 2015
Pages 128-133
DOI: 10.5101/nbe.v7i3.p128-133
Cite this article:
Khanra K, Panja S, Choudhuri I, et al. Evaluation of Antibacterial Activity and Cytotoxicity of Green Synthesized Silver Nanoparticles Using Scoparia Dulcis. Nano Biomedicine and Engineering, 2015, 7(3): 128-133. https://doi.org/10.5101/nbe.v7i3.p128-133

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Received: 10 September 2015
Accepted: 29 September 2015
Published: 08 October 2015
© 2015 Kalyani Khanra, Sudipta Panja, Indranil Choudhuri, Anindita Chakraborty and Nandan Bhattacharyya.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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