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Research Article | Open Access

Development of Nanodrug for Treatment of Breast Cancer Using Mallotus Tetracoccus Leaves-Standardisation, Synthesis and Characterisation

Ramalakshmi Subbiah1,2, Krishnaswamy Muthuchelian1( )
Department of Bioenergy, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai – 625 021, India
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore-641003
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Abstract

In this study, an ecofriendly, green method for synthesis of silver nanoparticles (AgNP) has been developed using Mallotus tetracoccus (MT) leaves as a reducing agent. The formation of AgNPs was standardised at pH 7 and 60 ℃. UV-visible spectroscopy showed the high peak of absorption band at 420 nm. By atomic force microscopic (AFM) and scanning electron microscopic (SEM) observations, the size of the silver nanoparticles was found to be in the range of 46 to 100 nm, with an average size of 73 nm. The energy dispersive x-ray spectroscopic (EDX) profile of silver nanoparticles showed typical optical absorption peak approximately at 3 keV. FTIR spectroscopic study revealed that hydroxyl groups of phenols and carboxylic acids were involved in the formation of AgNPs. Through MTT assay, the cytotoxicity results showed that AgNPs were highly effective on human ductal breast carcinoma cell lines (T47D) (76.8 to 84.9%). Thus, the MT-synthesized NPs are said to possess significant anticancer activity on cancer cells and very less toxicity on normal cells, which suggests its further applications in medicine.

Keywords

Silver nanoparticlesGreen synthesisNanodrugAnticancer activityMallotus tetracoccus

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Nano Biomedicine and Engineering
Volume 8 Issue 2,
June 2016
Pages 82-90
DOI: 10.5101/nbe.v8i2.p82-90
Cite this article:
Subbiah R, Muthuchelian K. Development of Nanodrug for Treatment of Breast Cancer Using Mallotus Tetracoccus Leaves-Standardisation, Synthesis and Characterisation. Nano Biomedicine and Engineering, 2016, 8(2): 82-90. https://doi.org/10.5101/nbe.v8i2.p82-90

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Received: 13 May 2016
Accepted: 15 June 2016
Published: 20 June 2016
© 2016 Ramalakshmi Subbiah, Krishnaswamy Muthuchelian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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