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Research Article | Open Access

Human CIK Cells Loaded with Gold Nanoprisms as Theranostic Platform for Targeted Photoacoustic Imaging and Enhanced Immuno-Photothermal Combined Therapy

Jingjing Zhang1, Fangfang Xia1, Yao Yang1, Caixia Yue1, Chunlei Zhang1, Yuming Yang1( ), Lijun Ma3, Gabriel Alfranca1, Yanlei Liu1, Yafei Hou1, Weilin Jin1, Jian Ni1, Jesus Martinez de la Fuente1,4, Daxiang Cui1,2,3( )
Institute of Nano Biomedicine and Engineering, Shanghai Engineering Center for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China
National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China
Department of tumor, Shanghai Tong Ren Hospital attached to Shanghai Jiao Tong university, 786 Yuyuan Road, Shanghai 200050, P. R. China
Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, Zaragoza, 50018, Spain
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Abstract

How to enhance the therapeutic efficacy of human cytokine induced killers cell (CIK) has become a great challenge. Herein, we report for the first time that human CIK cells loaded with gold nanoprisms were successfully used for targeted photoacoustic imaging, enhanced immunotherapy and photothermal therapy of gastric cancer in vivo. Gold nanoprisms were synthesized and modified with PEG; human CIK cells were prepared and incubated with PEGylated gold nanoprisms (Au GNPrs), and then the effects of human CIK cells labeled with Au NPrs on gastric cancer MGC 803 cells were evaluated and further used for targeted photoacoustic imaging, immunotherapy and photothermal therapy of gastric cancer in vivo in mice models. Results showed that PEGylated Au NPrs could be uptaken high-efficiently by human CIK cells, resultant human CIK cells labeled with AuNPrs could inhibit the growth of gastric cancer MGC 803 cells actively by induced apoptosis and G1 phase arrest, and actively target and accumulate the tumor sites in gastric cancer-bearing nude mice. Enhanced synergistic therapeutic efficacy was demonstrated with the maximal inhibition of tumor through a combination of CIK cells-based immunotherapy for three days and then a continuous gold nanoprisms-based photothermal therapy. In conclusion, human CIK cells labeled with PEGylated Au NPrs can target gastric cancer cells in vivo, enhance immunotherapy and photothermal therapy efficacy, and have a great potential in applications such as targeted imaging, immunotherapy and photothermal therapy of gastric cancer in the near future.

Keywords

Photoacoustic imagingPhotothermal therapyGastric cancerCytokine factorsGold nanoprismCIK cellTargeted imaging

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Nano Biomedicine and Engineering
Volume 8 Issue 3,
September 2016
Pages 112-127
DOI: 10.5101/nbe.v8i3.p112-127
Cite this article:
Zhang J, Xia F, Yang Y, et al. Human CIK Cells Loaded with Gold Nanoprisms as Theranostic Platform for Targeted Photoacoustic Imaging and Enhanced Immuno-Photothermal Combined Therapy. Nano Biomedicine and Engineering, 2016, 8(3): 112-127. https://doi.org/10.5101/nbe.v8i3.p112-127

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Received: 29 June 2016
Accepted: 04 July 2016
Published: 11 July 2016
© 2016 Jingjing Zhang, Fangfang Xia, Yao Yang, Caixia Yue, Chunlei Zhang, Yuming Yang, Lijun Ma, Gabriel Alfranca, Yanlei Liu, Yafei Hou, Weilin Jin, Jian Ni, Jesus Martinez de la Fuente and Daxiang Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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