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Research Article | Open Access

EGFR Antibody Conjugated Bimetallic Au@Ag Nanorods for Enhanced SERS-Based Tumor Boundary Identification, Targeted Photoacoustic Imaging and Photothermal Therapy

Shouhui Chen1,2, Chenchen Bao1, Chunlei Zhang1, Yao Yang1, Kan Wang1, Bhaskara V. Chikkaveeraiah2,3, Zhihua Wang1,2, Xinglu Huang2, Fei Pan1, Kun Wang1, Xiao Zhi1, Jian Ni1( ), Jesus M. de la Fuente1,5( ), Jie Tian4
Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Shanghai Engineering Center for Intelligent Diagnosis and Treatment Instrument, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA
Microfabrication and Microfluidics Unit, Biomedical Engineering and Physical Science Shared Resource, National Institutes of Health (NIH), Bethesda, Maryland 20892, USA
Intelligent Medical Research Center, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, Zaragoza, 50018, Spain
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Abstract

The development of multifunctional nanoprobes for simultaneous targeted imaging, tumor boundary identification and photothermal therapy of gastric cancer has become a great challenge. Herein, EGFR monoclonal antibody-conjugated Au@Ag nanorods decorated with DTNB nanoprobes (5,5'-Dithiobis-(2-nitrobenzoic acid)) (EGFR-Au@Ag NR nanotags) were prepared and characterized. Their biocompatibility was analyzed by MTT assay. In vitro studies show that EGFR-Au@Ag NR nanotags own good biocompatibility and capability of targeting and entering into gastric cancer MGC803 cells, silver nano-film layer on the surface of gold nanorods remarkably enhance surface-enhanced rama spectra (SERS) signal, enhanced photoacoustic imaging efficacy and photothermal conversion efficiency of gold nanorods. In vivo studies show that prepared nanotags could target actively gastric cancer cells at 2h post-injection, and distributed in the tumor site, exhibited enhanced SERS signals to display clearly tumor boundary, enhanced photoacoustic imaging to display clearly tumor boundary. Under 1w/cm2 laser irradiation, tumor growth was remarkably inhibited by local photothermal therapy. In conclusion, high performance EGFR-antibody conjugated Au @ Ag nanorod-DTNB nanoprobes exhibit great potential in applications such as targeted photoacoustic imaging, simultaneous tumor boundary identification and selective photothermal therapy of gastric cancer in the near future.

Keywords

Photoacoustic imagingPhotothermal therapyAu@Ag nanorodsSERS imagingGastric cancerTumor boundary

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Nano Biomedicine and Engineering
Volume 8 Issue 4,
December 2016
Pages 315-328
DOI: 10.5101/nbe.v8i4.p315-328
Cite this article:
Chen S, Bao C, Zhang C, et al. EGFR Antibody Conjugated Bimetallic Au@Ag Nanorods for Enhanced SERS-Based Tumor Boundary Identification, Targeted Photoacoustic Imaging and Photothermal Therapy. Nano Biomedicine and Engineering, 2016, 8(4): 315-328. https://doi.org/10.5101/nbe.v8i4.p315-328

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Received: 22 December 2016
Accepted: 27 December 2016
Published: 30 December 2016
© 2016 Shouhui Chen, Chenchen Bao, Chunlei Zhang, Yao Yang, Kan Wang, Bhaskara V. Chikkaveeraiah, Zhihua Wang, Xinglu Huang, Fei Pan, Kun Wang, Xiao Zhi, Jian Ni, Jesus M. de la Fuente, and Jie Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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