AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Biomedicine and Engineering Article
PDF (1.7 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

Interaction of Colloidal Gold Nanoparticles with Protein

Department of Chemistry, Faculty of Science, University of Kufa, Najf, Iraq
Show Author Information

Abstract

The interaction between nanoparticles (NPs) with biomaterials has many applications. In the present research, the interaction between colloidal gold nanoparticles and human protein, human chorionic gonadotropin (hCG) was studied to find out the changes in the structure of protein at the presence of colloidal gold nanoparticles and the quantity of protein that colloidal gold nanoparticles absorbed on the hCG surface. Colloidal gold nanoparticles were synthesized through the reduction of HAuCl4 using ammonium hydroxide. The gold nanoparticles were estimated at 20 nm in diameter. The results showed that there was a significant adsorption of colloidal gold nanoparticles absorbed on the hCG surface. Sips equation was used to express the absorption of isotherms and to calculate the absorption parameters and thermodynamic constant. Fluorescence study indicated a mild change in the tertiary structure near the microenvironment of the aromatic amino acids tyrosine and phenyl alanine, which was due to the interaction forces between the colloidal gold nanoparticles and hCG protein that affected the environment. It can be concluded that the colloidal gold nanoparticles had effects on the tertiary structure of proteins and affected the availability of hCG concentration.

Keywords

GoldNanoparticlesAbsorptionProteinhCG

References

[1]

A. Albanese, P.S. Tang, and W.C.W. Chan, The effect of nanoparticle size, shape, and surface chemistry on biological systems. Annu. Rev. Biomed. Eng., 2012, 14: 1-16.
Crossref Google Scholar
[2]

S. Swain, Nanoparticles for cancer targeting: Current and future directions. Curr Drug Deliv., 2016, 13(8): 1290-1302.
Crossref Google Scholar
[3]

X.L. Loh, Utilising inorganic nanocarriers for gene delivery. Biomat. Sci., 2016, 4(1): 70-86.
Crossref Google Scholar
[4]

A.R. Fernandes, Multifunctional gold-nanoparticles: A nanovectorization tool for the targeted delivery of novel chemotherapeutic agents. J. Contr. Release, 2017, 245: 52-61.
Crossref Google Scholar
[5]

M.A. Safwat, Gold nanoparticles enhance 5-fluorouracil anticancer efficacy against colorectal cancer cells. Inter. J. Pharmaceutics, 2016, 513(1–2): 648-658.
Crossref Google Scholar
[6]

Z. Li, Recent advances of using hybrid nanocarriers in remotely controlled therapeutic delivery. Small, 2016, 12(35): 4782-4806.
Crossref Google Scholar
[7]

E. Engel, A. Michiardi, M. Navarro, et al., Nanotechnology in regenerative medicine: the materials side. Trends Biotechnol., 2008, 26: 39-47.
Crossref Google Scholar
[8]

A. Patzelt, Do nanoparticles have a future in dermal drug delivery? J. Contr. Release, 2017, 246: 174-182.
Crossref Google Scholar
[9]

W. Zhang, pH and near-infrared light dual-stimuli responsive drug delivery using DNA-conjugated gold nanorods for effective treatment of multidrug resistant cancer cells. J. Contr. Release, 2016, 232: 9-19.
Crossref Google Scholar
[10]

E. Ye, An experimental and theoretical investigation of the anisotropic branching in gold nanocrosses. Nanoscale, 2016, 8(1): 543-552.
Crossref Google Scholar
[11]

R. Shukla, Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir, 2005, 21(23): 10644-10654.
Crossref Google Scholar
[12]

D. Gidaspow, V. Jiradilok, Nanoparticle gasifier fuel cell for sustainable energy future. J Power Source, 2007, 166: 400-410.
Crossref Google Scholar
[13]

I.L. Medintz, H.T. Uyeda, E.R. Goldman, et al. Quantum dot bioconjugates for imaging, labeling and sensing. Nat Mater., 2005, 4: 435-446.
Crossref Google Scholar
[14]

C. Corot. P. Robert, J.M. Idée, et al., Recent advances in iron oxide nanocrystal technology for medical imaging. Adv. Drug Deliv. Rev., 2006, 58: 1471–1504.
Crossref Google Scholar
[15]

K. Huang, Q. Dou, X.J. Loh, Nanomaterial mediated optogenetics: opportunities and challenges. RSC Advances, 2016, 6(65): 60896-60906.
Crossref Google Scholar
[16]

M.M. Teo, D.J. Young, and X.J. Loh, Magnetic anisotropic particles: Toward remotely actuated applications. Part. Part. Sys. Char., 2016, 33(10): 709-728.
Crossref Google Scholar
[17]

D.M. Mariagrazia, S. Shaharum, and A.R. Khairunisak, Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation. Inter. J. Nanomed., 2010, 5: 37-49
Crossref Google Scholar
[18]

B. Steven, K. Galina, and O. John, Metabolism of hCG and hLH to multiple urinary forms. Mol. Cellular Endocrinol., 1996, 125: 121-131.
Crossref Google Scholar
[19]

G. Kovalevskaya, O. Genbacev, and S.R. Fisher, Trophoblast origin of hCG isoforms: cytotrophoblasts are the primary source of choriocarcinoma-like hCG. Mol. Cellular Endocrinol., 2002, 194: 147-155.
Crossref Google Scholar
[20]

O.P. Bahl, R.B. Carlsen, Human chorionic gonadotrophin: Amino acid sequences of the α and β subunits. J Biol Chem., 1975, 250: 5247-5253.
Crossref Google Scholar
[21]

F.J. Morgan, S. Birken, and R.E. Canfield, The amino acid sequence of human chorionic gonadotropin. The α-subunit and the β- subunit. Endocrinology, 1975, 250: 5247-5255.
Crossref Google Scholar
[22]

L.A. Cole, Biological functions of hCG and hCG-related Molecules. Cole Reproductive Biolo. Endocrinology, 2010, 8: 102-112.
Crossref Google Scholar
[23]

C. Stock, S.F. Pedersen, Roles of pH and the Na+/H+ exchanger NHE1 in cancer: From cell biology and animal models to an emerging translational perspective? Semin Cancer Biol., 2017, 43: 5-16.
Crossref Google Scholar
[24]

K. Shameli, M.B. Ahmad, P. Shabanzadeh, et al., Effect of Curcuma longa tuber powder extract on size of silver nanoparticles prepared by green method. Res. Chem. Intermed., 2014, 40 (3): 1313-1325.
Crossref Google Scholar
[25]

I. Lynch, C.T. edervall, M. Lundqvist, et al., The nanoparticle-protein complex as a biological entity; a complex fluids and surface science challenge for the 21st century. Adv. Colloid Interface Sci., 2007, 134–135: 167-174.
Crossref Google Scholar
[26]

K. Shameli, M.B. Ahmad, EAJ Al-Mulla, et al., Antibacterial effect of silver nanoparticles on talc composites. Res. Chem. Intermed., 2015, 41(1): 251-263.
Crossref Google Scholar
[27]

Z. Zhang, Y. Zhang, Method of calculating the thermodynamic parameters from some isothermal absorption models. Acta of Northwest Sci-Tech University of Agriculture and Forestry, 1998, 26(2): 94-98.
Google Scholar
[28]

A.J. Haider, M.R. Mohammed, Synthesis of silver nanoparticle decorated carbon nanotubes and its antimicrobial activity against growth of bacteria. Rendiconti Lincei, 2014, 25(3): 403-407.
Crossref Google Scholar
[29]

M. Epple, K. Ganesan, R. Heumann R., Klesing at al., Application of calcium phosphate nanoparticles in biomedicine, J. Mater. Chem., 2010, 20: 18–23.
Crossref Google Scholar
[30]

P. Neogi, J.C. Wang, Stability of two-dimensional growth of a packed body of proteins on a solid surface. Langmuir, 2011, 9: 5347-553.
Crossref Google Scholar
[31]

S. Umoren, U. Etim, A. Israel, Adsorption of methylene blue from industrial effluent using poly (vinyl alcohol). Mater J. Environ. Sci., 2013, 4(1): 75-86.
Google Scholar
[32]

L. Wang, W. Zhao, W. Tan, Bioconjugated silica nanoparticles: Development and applications. Nano Res., 2008, 1: 99-115.
Crossref Google Scholar
[33]

Q. Liu, T. Zheng, Equilibrium isotherms and kinetics modeling of methylene blue adsorption on agricultural wastes-based activated carbons. Surf. Sci., 2010, 256: 3309-3315.
Crossref Google Scholar
[34]

P. Kumar, S. Ramalingam, and K. Sathishkumar, Adsorption characteristics of methylene blue onto the N-succinyl-chitosan-g-polyacrylamide/attapulgite composite. Chem. Eng., 2011, 28: 149–155.
Google Scholar
[35]

J. Toth, Adsorption: theory, modeling, and analysis Surfactant, Marcel Dekker, Sci. Ser., 2002, 107(50): 971-983.
Google Scholar
[36]

M. De, S. Rana, and V.M. Rotello, Nickel-ion-mediated control of the stoichiometry of His-tagged protein/nanoparticle interactions. Macromol. Biosci., 2009, 9: 174-178.
Crossref Google Scholar
[37]

S. Vaitheeswaran, A. Garcia, Protein stability at a carbon nanotube interface. J. Chem. Phys., 2011, 134: 12-24.
Crossref Google Scholar
[38]

S. Mitragotri, J. Lahann, Physical approaches to biomaterial design. Nature Mater., 2009, 8: 15-23.
Crossref Google Scholar
[39]

Z. Adamczyk, J. Barbasz, and M. Cieśla, Mechanisms of fibrinogen adsorption at solid substrates. Langmuir, 2011, 27(11): 6868-6878.
Crossref Google Scholar
[40]

M.R. Eftink, Methods of biochemical analysis. John Wiley, NY, 1991: 127-205.
[41]

J.R. Lakowicz, Principles of fluorescence spectroscopy. Kluwer-Plenum, NY, 1999: 69-71.
Crossref
Nano Biomedicine and Engineering
Volume 9 Issue 4,
December 2017
Pages 298-305
DOI: 10.5101/nbe.v9i4.p298-305
Cite this article:
Radhi SW. Interaction of Colloidal Gold Nanoparticles with Protein. Nano Biomedicine and Engineering, 2017, 9(4): 298-305. https://doi.org/10.5101/nbe.v9i4.p298-305

469

Views

16

Downloads

2

Crossref

2

Scopus

Altmetrics
Received: 02 November 2017
Accepted: 28 November 2017
Published: 25 December 2017
© Sami Waheed Radhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Return