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Research Article | Open Access

NRAMP1 Gene Polymorphism and Susceptibility to Cutaneous Leishmaniasis in Iraq

Ghada Basil Alomashi, Hasan Raheem Khudhur( )
College of Medicine, University of Al-Qadisiyah, Iraq
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Abstract

Cutaneous leishmaniasis (CL) is a vector-borne disease and endemic in most regions of Iraq, especially in the regions with poor populations. Natural resistance associated macrophage protein 1 (NRAMP1) gene plays an essential role in susceptibility to CL and disease pathology. This study aimed to study the polymorphism in NRAMP1 gene, and tried to identify an association between gene variants and susceptibility to CL infection in Iraqi population / AL-Muthanna province. Samples of peripheral blood were collected from 60 patients with CL and 32 apparently healthy controls. NRAMP1 (D543N) polymorphism was detected in patients and control groups by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The results indicated a statistically significant difference in genotype distribution between CL cases and healthy controls (p = 0.036), and the results indicated that genetic variations of D543N were not associated with susceptibility to CL infection, and the frequency of allele A was greater in controls than in patients with statistical significance of p = 0.01.

Keywords

Cutaneous leishmaniasisIraqNRAMP1 polymorphismsD543N

References

[1]

J. Postigo, Leishmaniasis in the world health organization Eastern Mediterranean region. International Journal of Antimicrobial Agents, 2016, 36: 62-65.
Crossref Google Scholar
[2]

A.M. AL-Samaria, H.S. Al-Obaidi, Cutaneous Leishmaniasis in Iraq. J. Infect. Developing Countries, 2009, 3(2): 123-129.
Crossref Google Scholar
[3]

P. Tripathi, V. Singh, and S. Naik, Immune response to Leishmania: Paradox rather than paradigm. FEMS Immunol Med Microbiol, 2007, 51(2): 229-242.
Crossref Google Scholar
[4]

V. Mulero, S. Searle, J.M. Blackwell, et al., Solute carrier 11a1 (Slc11a1; formerly Nramp1) regulates metabolism and release of iron acquired by phagocytic, but not transferrin- receptor-mediated, iron uptake. Biochem J, 2002, 363(1): 89-94.
Crossref Google Scholar
[5]

C.A.Taype, J.C.Castro, R.A. Accinelli, et al., Association between SLC11A1 polymorphisms and susceptibility to different clinical forms of tuberculosis in the Peruvian population. Infection, Genetics, and Evolution, 2006, 6(5):361-367.
Crossref Google Scholar
[6]

F. Canonne-Hergaux, J. Calafat, E. Richer, et al., Expression and subcellular localization of NRAMP1 in human neutrophil granules. Blood, 2002, 100: 268-275.
Crossref Google Scholar
[7]

J.H. Kim, S.Y. Lee, S.H. Lee, et al, NRAMP1 genetic polymorphisms as a risk factor of tuberculous pleurisy. Int J Tuberc Lung Dis, 2003, 7: 370-375.
Google Scholar
[8]

J.M. Blackwell, S. Searle, T. Goswaml, et al., Understanding the multiple functions of NRAMP1. Microbes Infect, 2009, 2(3): 317-321.
Crossref Google Scholar
[9]

P. Courville, R. Chaloupka, and M.F. Cellier, Recent progress in structure-function analyses of nramp proton-dependent metal-ion transporters. Biochem Cell Biol, 2006, 84(6): 960-978.
Crossref Google Scholar
[10]

M. Fattahi-Dolatabadi, I.T. Mousavi, H. Mohammadi-Barzelighi, et al., NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation of host susceptibility and treatment outcome. J Vector Borne Dis, 2016, 53: 257-263
Google Scholar
[11]

A.M. Da-Cruz, R. Bittar, M, Mattos, et al., T-cell-mediated immune responses in patients with cutaneous or mucosal leishmaniasis: long-term evaluation after therapy. Clin Diagn Lab Immunol, 2002, 9: 251-256.
Crossref Google Scholar
[12]

R Development Core Team, R, A language, and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2008.
[13]

A. Ortiz-Flores, G. De la Rosa-Lopez, B. Zavaleta-Villa, et al., Association of leishmaniasis with TNF alpha promoter and SLC11A1 gene polymorphisms in patients of two endemic areas in Mexico. Microbes Infect, 2015, 17(5): 387-394.
Crossref Google Scholar
[14]

M.Sophie, A. Hameed, A. Muneer, et al., SLC11A1 polymorphisms and host susceptibility to cutaneous leishmaniasis in Pakistan Mariam. Parasites and Vectors, 2017, 10: 12
Crossref Google Scholar
[15]

T.N. Samaranayake, S.D. Fernando, and V.H. Dissanayake, Candidate gene study of susceptibility to cutaneous leishmaniasis in Sri Lanka. Trop Med Int Health, 2010, 15(5): 632-638.
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 9 Issue 4,
December 2017
Pages 333-336
DOI: 10.5101/nbe.v9i4.p333-336
Cite this article:
Alomashi GB, Khudhur HR. NRAMP1 Gene Polymorphism and Susceptibility to Cutaneous Leishmaniasis in Iraq. Nano Biomedicine and Engineering, 2017, 9(4): 333-336. https://doi.org/10.5101/nbe.v9i4.p333-336

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Received: 01 November 2017
Accepted: 25 December 2017
Published: 29 December 2017
© Ghada Basil Alomashi, and Hasan Raheem Khudhur.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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