Effects of Effective Microorganisms Culture on the Quality of Puffer Fish Meat
, Xue TANG1 (
)Abstract
The effects of effective microorganisms (EM) culture (EM bacteria culture mode) and common culture mode on nutrient composition, texture characteristics, and characteristic flavor of puffer fish meat were compared and analyzed. The results showed that the relative content of essential amino acids (EAA) was higher in EM bacteria culture mode compared with the common culture mode, and the ratio of EAA to total amino acids and EAA to nonessential amino acids was significantly increased (P < 0.05). The contents of K, Na, and P elements were higher. Among liver fatty acids, the relative content of eicosapentaenoic acid (2.65%), docosahexaenoic acid (6.52%) and Σω-3 polyunsaturated fatty acid (9.91%) were higher. EM culture mode could improve the texture characteristics of fish meat, showing that hardness was decreased, adhesion and cohesion were increased. In addition, in the terms of characteristic flavor, the contents of amami amino acids and sweet amino acids in fish meat of EM bacteria culture mode group were higher, at the same time, the content of fishy odor substances trimethylamine (TMA) and trimethylamine oxide (TMAO) was significantly reduced (P < 0.05). The results of this study showed that the nutrition value of puffer fish under the EM bacteria culture mode was higher, the composition of amino acids and fatty acids was excellent, and the edible taste and flavor were better, which could provide valuable theoretical guidance for the application and promotion of the EM bacteria culture mode of puffer fish.
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References
LIN C, LI Q R, LIU D, et al. Recent research progress in tetrodotoxin detection and quantitative analysis methods[J]. Frontiers in Chemistry, 2024, 12: 1447312. DOI:10.3389/fchem.2024.1447312.
CHOMWONG S, CHAROENAPSRI W, AMPARYUP P, et al. Two host gut-derived lactic acid bacteria activate the proPO system and increase resistance to an AHPND-causing strain of Vibrio parahaemolyticus in the shrimp Litopenaeus vannarnei[J]. Developmental and Comparative Immunology, 2018, 89: 54-65. DOI:10.1016/j.dci.2018.08.002.
CHEN D W, ZHANG M, SHRESTHA S. Compositional characteristics and nutritional quality of Chinese mitten crab (Eriocheir sinensis)[J]. Food Chemistry, 2006, 103(4): 1343-1349.
SOFIA B, ALESSANDRO D. A contemporary review of the relationship between red meat consumption and cardiovascular risk[J]. International Journal of Preventive Medicine, 2017, 8(1): 40. DOI:10.4103/ijpvm.IJPVM_206_16.
MEYER N M T, POHRT A, WERNICKE C, et al. Improvement in visceral adipose tissue and LDL cholesterol by high PUFA intake: 1-year results of the nutriact trial[J]. Nutrients, 2024, 16(7): 1057. DOI:10.3390/nu16071057.
TUTOR A, O’KEEFE E L, LAVIE C J, et al. Omega-3 fatty acids in primary and secondary prevention of cardiovascular diseases[J]. Progress in Cardiovascular Diseases, 2024, 84: 19-26. DOI:10.1016/j.pcad.2024.03.009.
CHEN X T, ZHANG J W, JIA Y D. Culture system impact on fatty acids and amino acids accumulation in tiger pufferfish (Takifugu rubripes)[J]. Aquaculture, 2024, 585: 740694. DOI:10.1016/j.aquaculture.2024.740694.
ZHONG C, NAKANISHI M, GENG J T, et al. Comparison of nonvolatile taste-active components in fish sauce produced from lizardfish Saurida wanieso viscera under different conditions[J]. Fisheries Science, 2015, 81(3): 581-590. DOI:10.1007/s12562-015-0865-8.
LIOE H N, APRIYANTONO A, TAKARA K, et al. Umami taste enhancement of MSG/NaCl mixtures by subthreshold L-α-aromatic amino acids[J]. Journal of Food Science, 2005, 70(7): s401-s405. DOI:10.1111/j.1365-2621.2005.tb11483.x.
TIKK M, TIKK K, TØRNGREN M A, et al. Development of inosine monophosphate and its degradation products during aging of pork of different qualities in relation to basic taste and retronasal flavor perception of the meat[J]. Journal of Agricultural and Food Chemistry, 2006, 54(20): 7769-7777. DOI:10.1021/jf060145a.
YUE J, ZHANG Y F, JIN Y F, et al. Impact of high hydrostatic pressure on non-volatile and volatile compounds of squid muscles[J]. Food Chemistry, 2016, 194: 12-19. DOI:10.1016/j.foodchem.2015.07.134.
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