Preparation and Properties of Passion Fruit Seed Oil Pickering Emulsion Stabilized by β-Lactoglobulin-Polyphenol Nanoparticles
Abstract
In this study, a passion fruit seed oil Pickering emulsion stabilized by β-lactoglobulin nanoparticles loaded with a ternary mixture of ferulic acid, quercetin and vanillic acid (β-LGCNPs) was prepared by an ultrasonic method. The particle size, stability, and microstructure, and antioxidant, digestibility, rheological properties and lipid oxidation products of Pickering emulsions prepared under varying conditions of nanoparticle concentration and oil phase ratio were investigated. The results showed that the Pickering emulsion was a stable oil-in-water (O/W) emulsion with β-LGCNPs adsorbed at the oil-water interface. The particle size of the emulsion was directly proportional to the particle concentration and inversely proportional to the oil phase ratio. The average particle size of the Pickering emulsion prepared at β-LGCNP concentration of 1.5% and 30% oil phase was (5.78 ± 0.10) μm. The emulsion had good stability under different ionic strength and pH conditions, showing stronger antioxidant properties than passion fruit seed oil. The free radical scavenging capacity was dependent on β-LGCNP concentration. After intestinal digestion for 2 h, the release rate of free fatty acids from the Pickering emulsion was (65.17 ± 1.52)%, which was 26.65% higher than that from passion fruit seed oil. The rheological results showed a shear-thinning phenomenon, indicating that the emulsion is a non-Newtonian fluid, and the elastic and viscous moduli increased with the increase in shear frequency. During 15 days of storage, the amount of lipid oxidation products including hydroperoxide and malondialdehyde (MDA), and the rate of lipid oxidation in the Pickering emulsion decreased. In summary, the Pickering emulsion stabilized with β-LGCNPs improved the stability, antioxidant activity, digestibility and lipid oxidation of passion fruit seed oil, which is conducive to expanding the application of passion fruit seed oil in the food field.
Keywords
References
FONSECA A M A, GERALDI M V, MARÓSTICA M R M Jr, et al. Purple passion fruit (Passiflora edulis F. edulis): a comprehensive review on the nutritional value, phytochemical profile and associated health effects[J]. Food Research International, 2022, 160: 111665. DOI:10.1016/j.foodres.2022.111665.
OLIVEIRA D A, MEZZOMO N, GOMES C, et al. Encapsulation of passion fruit seed oil by means of supercritical antisolvent process[J]. The Journal of Supercritical Fluids, 2017, 129: 96-105. DOI:10.1016/j.supflu.2017.02.011.
OLIVEIRA D A, ANGONESE M, GOMES C, et al. Valorization of passion fruit (Passiflora edulis sp.) by-products: sustainable recovery and biological activities[J]. The Journal of Supercritical Fluids, 2016, 111: 55-62. DOI:10.1016/j.supflu.2016.01.010.
LIM S, SALENTINIG S. Protein nanocage-stabilized Pickering emulsions[J]. Current Opinion in Colloid & Interface Science, 2021, 56: 101485. DOI:10.1016/j.cocis.2021.101485.
LESMES U, MCCLEMENTS D J. Structure-function relationships to guide rational design and fabrication of particulate food delivery systems[J]. Trends in Food Science & Technology, 2009, 20(10): 448-457. DOI:10.1016/j.tifs.2009.05.006.
WANG C Z, WU J H, WANG C H, et al. Advances in Pickering emulsions stabilized by protein particles: toward particle fabrication, interaction and arrangement[J]. Food Research International, 2022, 157: 111380. DOI:10.1016/J.FOODRES.2022.111380.
XIA T H, XUE C H, WEI Z H. Physicochemical characteristics, applications and research trends of edible Pickering emulsions[J]. Trends in Food Science & Technology, 2021, 107: 1-15. DOI:10.1016/j.tifs.2020.11.019.
ZEMBYLA M, LAZIDIS A, MURRAY B S, et al. Stability of water-in-oil emulsions co-stabilized by polyphenol crystalprotein complexes as a function of shear rate and temperature[J]. Journal of Food Engineering, 2020, 281: 109991. DOI:10.1016/j.jfoodeng.2020.109991.
SU J Q, GUO Q, CHEN Y L, et al. Utilization of β-lactoglobulin-(-)-epigallocatechin-3-gallate (EGCG) composite colloidal nanoparticles as stabilizers for lutein Pickering emulsion[J]. Food Hydrocolloids, 2020, 98: 105293. DOI:10.1016/j.foodhyd.2019.105293.
WANG S W, ZHENG L L, JI Y X, et al. Enhancing the antioxidant and antifungal properties of polyphenols through encapsulation in β-lactoglobulin nanoparticles using emulsification-evaporation technique[J]. LWT-Food Science and Technology, 2023, 185: 115198. DOI:10.1016/j.lwt.2023.115198.
ZHANG S Y, LI X L, ZHENG L L, et al. Encapsulation of phenolics in β-lactoglobulin: stability, antioxidant activity, and inhibition of advanced glycation end products[J]. LWT-Food Science and Technology, 2022, 162: 113437. DOI:10.1016/j.lwt.2022.113437.
YUE M, HUANG M Y, ZHU Z S, et al. Effect of ultrasound assisted emulsification in the production of Pickering emulsion formulated with chitosan self-assembled particles: stability, macro, and micro rheological properties[J]. LWT-Food Science and Technology, 2022, 154: 112595. DOI:10.1016/j.lwt.2021.112595.
ALEHOSSEINI E, JAFARI S M, TABARESTANI H S. Production of D-limonene-loaded Pickering emulsions stabilized by chitosan nanoparticles[J]. Food Chemistry, 2021, 354: 129591. DOI:10.1016/j.foodchem.2021.129591.
MENG X Y, LIU H, DONG X Y, et al. A soft Pickering emulsifier made from chitosan and peptides endows stimuli-responsiveness, bioactivity and biocompatibility to emulsion[J]. Carbohydrate Polymers, 2022, 277: 118768. DOI:10.1016/j.carbpol.2021.118768.
ALEHOSSEINI E, JAFARI S M, SHAHIRI T H. Production of D-limonene-loaded Pickering emulsions stabilized by chitosan nanoparticles[J]. Food Chemistry, 2021, 354: 129591. DOI:10.1016/j.foodchem.2021.129591.
MENGIBAR M, MIRALLES B, HERAS Á. Use of soluble chitosans in Maillard reaction products with β-lactoglobulin emulsifying and antioxidant properties[J]. LWT-Food Science and Technology, 2017, 75: 440-446. DOI:10.1016/j.lwt.2016.09.016.
LIU M, LIANG J M, JING C, et al. Preparation and characterization of Lycium barbarum seed oil Pickering emulsions and evaluation of antioxidant activity[J]. Food Chemistry, 2023, 405: 134906. DOI:10.1016/j.foodchem.2022.134906.
HUANG M G, WANG Y, AHMAD M, et al. Fabrication of Pickering high internal phase emulsions stabilized by pecan protein/xanthan gum for enhanced stability and bioaccessibility of quercetin[J]. Food Chemistry, 2021, 357: 129732. DOI:10.1016/j.foodchem.2021.129732.
WANG J, ZHANG L L, TAN C, et al. Pickering emulsions by regulating the molecular interactions between gelatin and catechin for improving the interfacial and antioxidant properties[J]. Food Hydrocolloids, 2022, 126: 107425. DOI:10.1016/j.foodhyd.2021.107425.
MING L S, WU H L, LIU A, et al. Evolution and critical roles of particle properties in Pickering emulsion: a review[J]. Journal of Molecular Liquids, 2023, 388: 122775. DOI:10.1016/j.molliq.2023.122775.
LIU X, GUO J, WAN Z L, et al. Wheat gluten-stabilized high internal phase emulsions as mayonnaise replacers[J]. Food Hydrocolloids, 2018, 77: 168-175. DOI:10.1016/j.foodhyd.2017.09.032.
ZHANG X, LIANG H S, LI J, et al. Fabrication of processable and edible high internal phase Pickering emulsions stabilized with gliadin/sodium carboxymethyl cellulose colloid particles[J]. Food Hydrocolloids, 2022, 128: 107571. DOI:10.1016/j.foodhyd.2022.107571.
WANG R, ZHOU J. Waxy maize starch nanoparticles incorporated tea polyphenols to stabilize Pickering emulsion and inhibit oil oxidation[J]. Carbohydrate Polymers, 2022, 296: 119991. DOI:10.1016/j.carbpol.2022.119991.
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