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Research Article | Open Access

Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables

Bin Lia, Hanling Wanga, Jianguo Xua, Wei Qua, Li Yaoa( ), Bangben Yaoa,b, Chao Yanb, Wei Chena( )
Engineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, China
Anhui Provincial Institute of Product Quality Supervision and Inspection, Hefei 230051, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Rapid detection of target foodborne pathogens plays more and more significant roles in food safety, which requires the efficiency, sensitivity, and accuracy. In this research, we proposed a new strategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture. The designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min, resolving the drawbacks of traditional random sampling protocols. After simple and convenient ultrasonication, samples can be directly amplified at 39 ℃ in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min. Finally, satisfied results have been achieved within 40 min, which greatly improve the efficiency while the accuracy is also guaranteed. Furthermore, all detection steps can be completed under instrument-free conditions. This method will hold great promise for target pathogen detection in the resource-limited district, or for emergency on-site identification.

Keywords

Rapid detectionVegetablesSalmonellaFiltration enrichmentCulture-free detectionEnzymatic recombinase amplification (ERA)Lateral flow strip (LFS)Food safety

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Food Science and Human Wellness
Volume 12 Issue 4,
July 2023
Pages 1167-1173
DOI: 10.1016/j.fshw.2022.10.042
Cite this article:
Li B, Wang H, Xu J, et al. Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables. Food Science and Human Wellness, 2023, 12(4): 1167-1173. https://doi.org/10.1016/j.fshw.2022.10.042

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Received: 09 September 2021
Revised: 19 October 2022
Accepted: 03 January 2022
Published: 18 November 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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