Rapid detection of target foodborne pathogens plays more and more significant roles in food safety, which requires the efficiency, sensitivity, and accuracy. In this research, we proposed a new strategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture. The designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min, resolving the drawbacks of traditional random sampling protocols. After simple and convenient ultrasonication, samples can be directly amplified at 39 ℃ in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min. Finally, satisfied results have been achieved within 40 min, which greatly improve the efficiency while the accuracy is also guaranteed. Furthermore, all detection steps can be completed under instrument-free conditions. This method will hold great promise for target pathogen detection in the resource-limited district, or for emergency on-site identification.
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Article type
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Open Access
Research Article
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Food Science and Human Wellness 2023, 12 (4): 1167-1173
Published: 18 November 2022
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