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Review Article | Open Access

Culture models produced via biomanufacturing for neural tissue-like constructs based on primary neural and neural stem cells

Wei Chen1,2,3,§, Ke Gai1,2,3,§, Feng Lin1,2,3, Wei Sun1,2,3,4, Yu Song1,2,3( )
Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China
Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, China
"Biomanufacturing and Engineering Living Systems" Innovation International Talents Base (111 Base), Beijing 100084, China
Department of Mechanical Engineering, Drexel University, Philadelphia, PA, USA

§ These authors contributed equally to this work.

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Abstract

Neural tissue-like constructs have important application potential in both neural tissue regeneration and individual medical treatment due to the ideal bioenvironment they provide for the growth of primary and stem cells. The biomaterials used in three-dimensional (3D) biomanufacturing techniques play a critical role in bioenvironment fabrication. They help optimize the manufacturing techniques and the long-term environment that supports cell structure and nutrient transmission. This paper reviews the current progress being made in the biomaterials utilized in neural cell cultures for in vitro bioenvironment construction. The following four requirements for biomaterials are evaluated: biocompatibility, porosity, supportability, and permeability. This study also summarizes the recent culture models based on primary neural cells. Furthermore, the biomaterials used for neural stem cell constructs are discussed. This study’s results indicate that compared with traditional two-dimensional (2D) cultures (with minimal biomaterial requirements), modulus 3D cultures greatly benefit from optimized biomaterials for long-term culturing.

Keywords

biomaterialsneural stem cellsprimary neural cellsneural tissue-like constructs3D bioprinting

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Brain Science Advances
Volume 7 Issue 4,
December 2021
Pages 220-238
DOI: 10.26599/BSA.2021.9050021
Cite this article:
Chen W, Gai K, Lin F, et al. Culture models produced via biomanufacturing for neural tissue-like constructs based on primary neural and neural stem cells. Brain Science Advances, 2021, 7(4): 220-238. https://doi.org/10.26599/BSA.2021.9050021

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Received: 29 November 2021
Revised: 20 December 2021
Accepted: 03 January 2022
Published: 10 January 2022
© The authors 2021.

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