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Research Article | Open Access

Co-immunolocalization of Disc1 and Gas7 protein in adult mice brain

Unit of Biochemistry, Department of Zoology, University of Madras, Chennai, Tamil Nadu, India
Department of Neurology, University of California Los Angeles, Los Angeles, California, U.S.A.
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Abstract

Objective:

The aim of the present study was to check the potential interaction of two neurodevelopmental proteins, Disc1 and Gas7, in the adult mice brain.

Methods:

Twenty-four male Swiss albino mice were used for the study. The mice were 12 weeks old in the beginning of the experiment. Immunohistochemistry and co-immunofluorescence were performed on the coronal sections of mice brain and immunoblotting and co-immunoprecipitation were done on the whole brain lysate.

Results:

Data from immunohistochemistry and co-immunofluorescence indicate the occurrence and co-localization of Disc1 and Gas7 proteins in soma and projections of the brain cells. Immunostaining was observed in cerebral cortex, hypothalamus, midbrain, pons, medulla oblongata and CA3 of hippocampus of the brain. The data from Immunoblotting and co-immunoprecipitation validates the presence and interaction of Disc1 and Gas7 protein in whole brain lysate.

Conclusion:

Data indicates the potential interaction of Disc1 and Gas7 protein in adult brain. The study highlights the need for further research on Disc1-Gas7 protein interaction in brain development and neuro-disorders.

Keywords

Disc1Gas7co-immunolocalizationco-immunofluorescenceco-immunoprecipitation

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Brain Science Advances
Volume 8 Issue 1,
March 2022
Pages 70-77
DOI: 10.26599/BSA.2022.9050010
Cite this article:
Kumar U. Co-immunolocalization of Disc1 and Gas7 protein in adult mice brain. Brain Science Advances, 2022, 8(1): 70-77. https://doi.org/10.26599/BSA.2022.9050010

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Received: 25 February 2022
Revised: 19 March 2022
Accepted: 22 March 2022
Published: 22 May 2022
© The authors 2022.

This article is published with open access at journals.sagepub.com/home/BSA

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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