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Gold nanostructure can be manufactured in many forms, such as spherical by using simple chemical method. X-ray powder diffraction (XRD) pattern of gold nanoparticles (AuNPs) was derived from a 400-μL trisodium citrate solution. The diffraction peaks corresponding to the diffraction planes of (111), (200), and (220) were all indexed to the gold with a face-centered cubic structure. The lattice constant calculated from the XRD pattern is 4.078 Å, which matches the conventional cubic gold metal diffraction pattern well (Pattern card number (04-784)). The results of scanning electronic microscope show that the biological nanoparticles of gold have asymmetric shapes and different sizes grouped as a circular particle. It was observed that the fashioning of AuNPs increased with increase in the concentration of [6]-gingerol waste extracts. The viability of FTC-133 cells treated for 48 h with various concentrations of [6]-gingerol extract coated with AuNPs (12.5–400 g/mL) was used to determine the cytotoxicity. A dose-dependent reduction in FTC-133 cell viability caused by [6]-gingerol extract capped with Au NPs was substantial (P < 0.05), reaching 75% cell mortality at 400 g/mL; the IC50 was 90.5%.
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