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Research Article | Open Access

Preparation of Magnesium Oxide Nanoparticles and Study Its Loaded With Recombinant Human Erythropoietin alfa Drug

Fatin Fadhel Mohammed Al-Kazazz1, Shaimaa Hamed Jaber1, Asma Hadi Mohammed2, Amer Hasan Abdullah1, Mustafa M. Kadhim3, Ameer Radhi Sultan1( )
Department of Chemistry, College of Science, Al-Mustansiriyah University, Iraq
Department of physics, College of Science, Al-Mustansiriyah University, Iraq
Department of Dentistry, Kut University College, Iraq
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Abstract

Loading rHuEPO-alpha drug on the surface of magnesium oxide nanoparticles further improves the effect of the drug as a treatment for anemia. After preparation of magnesium oxide nanoparticles by precipitation method, it was diagnosed by X-ray diffraction and Transmission Electron Microscopy. the loaded was diagnosed by Docking program, UV-Visible and Transmission Electron Microscopy. The study of LD50 was for thirty male mice. Magnesium Oxide nanoparticles had an average crystallite size of 8.42 nm. oral LD50 test was 896 mg/kg. Docking results presented a high energy binding with force linkage due to the link of the oxygen atom of MgO NPs with three hydrogen bonds of amino acids in the rHuEPO-alpha structure. The UV-Visible result was certain that all rHuEPO-alpha drug was loaded on the surface of MgO NPs. TEM shows that the average particle size was 9.94 nm after loaded of the rHuEPO-alpha drug on MgO NPs while was 9 nm before the loaded. Magnesium oxide nanoparticles may exhibit an effect on increasing the efficacy of rHuEPO-alpha after the loaded, leading to elevated hemoglobin and reduced anemia disease.

Keywords

XRDDockingloaded rHuEPO-alpha drug on MgO NPsTEM and UV-Visible

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Nano Biomedicine and Engineering
Volume 14 Issue 2,
June 2022
Pages 186-191
DOI: 10.5101/nbe.v14i2.p186-191
Cite this article:
Al-Kazazz FFM, Jaber SH, Mohammed AH, et al. Preparation of Magnesium Oxide Nanoparticles and Study Its Loaded With Recombinant Human Erythropoietin alfa Drug. Nano Biomedicine and Engineering, 2022, 14(2): 186-191. https://doi.org/10.5101/nbe.v14i2.p186-191

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Received: 12 April 2022
Accepted: 17 October 2022
Published: 17 November 2022
© Fatin Fadhel Mohammed Al-Kazazz, Shaimaa Hamed Jaber, Asma Hadi Mohammed, Amer Hasan Abdullah, Mustafa M.Kadhim, and Ameer Radhi Sultan.

This is an opena-ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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