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Research Article | Open Access

Conventional Multiplex PCR for Identifying Soil Transmitted Helminthes (Ascaris Lumbricoides, Trichuris Trichura and Ancylostoma Duodenale) in Fecal Specimens

Ghada Basi Ali Alomashi1, Hasan Raheem Khudhur2( ), Layla Safar Jebur3
Department of Microbiology and Parasitology, College of Medicine, University of Al-Qadisiyah, Diwaniya, Iraq
College of Health and Medical Techniques, Sawa University, Samawah, Iraq
Department of Clinical Laboratory Sciences, College of Pharmacy, University of Al-Qadisiyah, Diwaniya, Iraq
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Abstract

Ascaris lumbricoides, Trichuris trichiura, and Ancylostoma duodenale are soil-transmitted helminths (STHs) and medically neglected in Iraq country in spite of their effect on the public health. A cross-sectional study was performed in the Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Dewanyia Province, included 850 tool samples collected from patients who attended to the O & P Lab. General stool examination (GSE), direct wet mount method DWMM and Kato-Katz were using for diagnosis of STH infections through detecting the adult and the ovum of the helminthes. A conventional multiplex PCR assay was used for detection of STHs in fecal samples. Based on microscopic examination, the results showed that 275/850 range among triple, double and single infection on other hand was 365/850 range among triple, double and single infection. In conclusions, the investigative sensitivity of DWMM is notable for STH; in exception, it is capable to identify patients with the intention of highest requirement of management, and therefore contributes to the universal target to reduce STH as a community healthiness trouble.

Keywords

Multiplex PCRAscais lumbricoidesTrichuris trichuraAncylostoma duodenaleFecal specimens

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Nano Biomedicine and Engineering
Volume 14 Issue 4,
December 2022
Pages 289-294
DOI: 10.5101/nbe.v14i4.p289-294
Cite this article:
Alomashi GBA, Khudhur HR, Jebur LS. Conventional Multiplex PCR for Identifying Soil Transmitted Helminthes (Ascaris Lumbricoides, Trichuris Trichura and Ancylostoma Duodenale) in Fecal Specimens. Nano Biomedicine and Engineering, 2022, 14(4): 289-294. https://doi.org/10.5101/nbe.v14i4.p289-294

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Received: 10 January 2022
Revised: 12 June 2022
Accepted: 29 December 2022
Published: 31 December 2022
© Ghada Basi Ali Alomashi, Hasan Raheem Khudhur and Layla Safar Jebur.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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