Ascaris lumbricoides, Trichuris trichiura, and Ancylostoma duodenale are soil-transmitted helminths (STHs) and medically neglected in Iraq country in spite of their effect on the public health. A cross-sectional study was performed in the Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Dewanyia Province, included 850 tool samples collected from patients who attended to the O & P Lab. General stool examination (GSE), direct wet mount method DWMM and Kato-Katz were using for diagnosis of STH infections through detecting the adult and the ovum of the helminthes. A conventional multiplex PCR assay was used for detection of STHs in fecal samples. Based on microscopic examination, the results showed that 275/850 range among triple, double and single infection on other hand was 365/850 range among triple, double and single infection. In conclusions, the investigative sensitivity of DWMM is notable for STH; in exception, it is capable to identify patients with the intention of highest requirement of management, and therefore contributes to the universal target to reduce STH as a community healthiness trouble.