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Research Article | Open Access

Spectrophotometric Determination of Vitamin Folic Acid B9 in Some Drugs Using 1, 2-Naphthoquine-4-Sulphonate (NQS)

Riyadh Radhi Al-Araji1( ), Muthana Saleh Mashkour1, Emad Abbas Jaffar Al-Mulla2( )
Department of Chemistry, Faculty of Science, University of Kufa, P.O. Box 21, An-Najaf 54001, Iraq
College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, 54003 Al-Kufa, Iraq
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Abstract

In this study, simple and sensitive spectrophotometric method for the determination of folic acid (VB9) in pharmaceutical formulations was reported. The proposed method was based on the reaction between folic acid and 1, 2-naphthoquine-4-sulphonate (NQS) at alkaline medium (pH 11) to form deep yellow product. Beer's law was obeyed in the range of 0.75-10.5 μg/mL of folic acid at maximum wavelength of 436 nm. Under optimized reaction conditions, linear regression equation of the calibration curve was y = 0.048 x + 0.038 (μg/mL) with a linear correlation coefficient of 0.9988. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.159 μg/mL and 0.531 μg/mL, respectively. The method was successfully applied to the determination of folic acid in pharmaceutical formulations.

Keywords

Folic acid (B9)SpectrophotometricPharmaceutical formulation1-2-naphthoquine-4-sulfonate (NQS)

References

[1]
S.H. Ural, Folic acid and pregnancy. Kid's health. American Family Hildren's Hospital, 2008: 11.
[2]

V. Sreeram, M.V.B. Rao, and S.R. Karumuri. A validated and stability indicating ultra high pressure liquid chromatographic method for folic acid in pharmaceutical preparation. Inter. J. Chem. Stud., 2013, 1: 1-11.
Google Scholar
[3]

J. Arcot, A. Shrestha, Folate: methods of analysis. Trends in Food Science and Technology, 2005, 6: 253-266.
Crossref Google Scholar
[4]
R.R. Eitenmiller, Jr. W.O. Lander, Vitamin analysis for the health and food sciences, 2nd edition. Taylor and Francis Group, 2008: 443-505.
[5]

S. Suzana, X.K. Lee, B. Siti Balkis, et al., Anaemia andcognitive function among Chinese elderly. Mal. J. Hath. Sci., 2004: In review.
Google Scholar
[6]

J. Lindenbaum, E.B. Healton, D.G. Savage, et al., Neuropsychiatric disorders caused by cobalamin deficiency in the absence of anemia or macrocytosis. N. Engl. J. Med., 1988, 318: 1720-1728.
Crossref Google Scholar
[7]

D.G. Savage, J. Lindenbaum, S.P. Stabler, et al., Sensitivity of serum methylmalonic acid and total homocysteine determinations for diagnosing cobalamin and folate deficiencies. Am. J. Med., 1994, 96: 239-246.
Crossref Google Scholar
[8]

E.R. Eichner, H.I. Pierce, and R.S. Hillman, Folate balance in dietary-induced megaloblastic anemia. N. Engl. J. Med., 1971, 284: 933-938.
Crossref Google Scholar
[9]

FAO/WHO, Folate and folic acid. Human vitamin and mineral requirements. Report of a Joint FAO/WHO Expert Consultation. FAO, 2002: 53-63.
Google Scholar
[10]
E.N. Whitney, C.B. Cataldo, and R.S. Rolfes, Understanding normal and clinical nutrition, West publishing Company, 1994.
[11]

A. Taherkhani, T. Jamali, H. Hadadzadeh, et al., ZnO nanoparticle-modified ionic liquid-carbon paste electrode for voltammetric determination of folic acid in food and pharmaceutical samples. Ionics, 2014, 20: 421-429.
Crossref Google Scholar
[12]

F. Xiao, C. Ruan, L. Liu, et al., Single-walled carbon nanotube-ionic liquid paste electrode for the sensitive voltammetric determination of folic acid. Sens. Actuators B. Chem., 2008, 134: 895-901.
Crossref Google Scholar
[13]

P. Nagaraja, R.A. Vasantha, and H.S. Yathirajan, Spectrophotometric determination of folic acid in pharmaceutical preparations by coupling reactions with iminodibenzyl or 3-aminophenol or sodium molybdatepyrocatechol. Anal. Biochem., 2002, 307: 316-321.
Crossref Google Scholar
[14]

Z. Song, X. Zhou, Chemiluminescence flow sensor for folic acid with immobilized reagents. Spectrochim. Acta. A. Mol. Biomol. Spectrosc., 2001, 57: 2567-2574.
Crossref Google Scholar
[15]

S. Zhao, H. Yuan, C. Xie, et al., Determination of folic acid by capillary electrophoresis with chemiluminescence detection. J. Chromatogr. A., 2006, 1107: 290-293.
Crossref Google Scholar
[16]

R. Amidžic´, J.S.C. Brboric´, O.A. Udina, et al., RpHPLC determination of vitamins, folic acid and B12 in multivitamin tablets. J. Serb. Chem. Soc., 2005, 70: 1229-1235.
Crossref Google Scholar
[17]

K. Ilango, P. Valentina, and K. S. Lakshmi, Spectrophotometric determination of Finasteride in tablet formulation. Indian J. Pharm. Sci., 2002, 64: 174-175.
Google Scholar
[18]

S.T. Ulu, A new spectrophotometric method for the determination of finasteride in tablets. Spectrochim. Acta. A. Mol. Biomol. Spectrosc., 2007, 67: 778-783.
Crossref Google Scholar
[19]

Q.M. Li, Z.J. Yang, Spectrophotometric determination of aminomethylbenzoic acid using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent. Spectrochim. Acta. A., 2007, 60: 656-661.
Crossref Google Scholar
[20]

M. Hasani, L. Yaghoubi, and H. Abdollahi, A kinetic spectrophotometric method for simultaneous determination of glycine and lysine by artificial neural networks. Anal. Biochem., 2007, 356: 74-81.
Crossref Google Scholar
[21]

I.A. Darwish, H.H. Abdine, S.M. Amer, et al., Spectrophotometric study for the reaction of fluvoxamine 1,2-naphthoquinone-4-sulphonate: kinetic, mechanism, and use for determination of fluvoxamine in its dosage forms. Spectrochim. Acta. A., 2009, 72: 897-902.
Crossref Google Scholar
[22]

A.A. Elbashir, A.A. Abir, M.A.A. Shazalia, et al., 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS) as an analytical reagent for the determination of pharmaceutical amine by spectrophotometry. Spectroscopy Review, 2012, 47: 219-232.
Crossref Google Scholar
[23]

J. Saurina, S. Hernandez-Cassou, Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate. J. Chromatogr. A., 1994, 676: 311.
Crossref Google Scholar
[24]

Y. Kobayashi, H. Kubo, and T. Kinoshita, Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography. Anal. Biochem., 1987, 160(2): 392-398.
Crossref Google Scholar
[25]

S.T. Sulaiman, D. Amin, Spectrophotometric determination of primaquine, chloroquine, 8-aminoquinoline and 4-aminoquinalidine with chloranil. Intern. J. Environ. Anal. Chem., 1985, 20: 313-317.
Crossref Google Scholar
[26]
P. Nagaraja, British pharmacopoeia. Her Majesty's Stationery Office, 2013: 23.
[27]
P. Job, Analytica chimica acta 16. Advanced physicochemical experiments, 2nd edition. Oliner and Boyd, 1964: 54.
[28]

L.G. Hargis, Analytical chemistry, principles and techniques. Analytical chemistry, principles and techniques. Prentice-Hall Inc., 1988: 424-427.
Google Scholar
[29]

M. Aida, A. Nouralla, and A. A. Elbashir, Spectrophotometric method for determination of primaquine in pharmaceutical formulations via derivatization with 1,2-naphthoquinone-4-sulfonate. Austin. J. Anal. Pharm. Chem., 2004, 1(4): 1019.
Google Scholar
[30]
J. Rose, Advanced physico-chemical experiments. Pittman, 1964: 67.
[31]
International Conference on Harmonization (ICH), Technical requirements for the registration of pharmaceuticals for human use, validation of analytical procedures. Text and methodology Q2, 2005.
[32]

T.A.R. Sulafa, A.E. Abdalla, E. Mohamed, et al., Development and validation of spectrophotometric method for determination of thiamine (VB1) in pharmaceutical formulations using 1, 2-naphthoquine-4-sulphonate (NQS). Enliven. Archive, 2005, 2(1): 5-6.
Google Scholar
[33]
H. Rajak, Validation of analytical procedure: Methodology. ICH harmonised tripartite guideline. CPMP, 1996: 1450.
[34]

V. Ravichandran, S. Shalini, K.M. Sundram, et al., Validation of analytical methods-strategies and importance. Int. J. Pharmacy and Pharm. Sci., 2010, 3: 18-22.
Google Scholar
Nano Biomedicine and Engineering
Volume 9 Issue 3,
September 2017
Pages 208-213
DOI: 10.5101/nbe.v9i3.p208-213
Cite this article:
Al-Araji RR, Mashkour MS, Al-Mulla EAJ. Spectrophotometric Determination of Vitamin Folic Acid B9 in Some Drugs Using 1, 2-Naphthoquine-4-Sulphonate (NQS). Nano Biomedicine and Engineering, 2017, 9(3): 208-213. https://doi.org/10.5101/nbe.v9i3.p208-213

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Received: 19 July 2017
Accepted: 14 August 2017
Published: 15 September 2017
© 2017 Riyadh Radhi Al-Araji, Muthana Saleh Mashkour, and Emad Abbas Jaffar Al-Mulla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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