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Research Article | Open Access

Spectrophotometric Determination of Vitamin Folic Acid B9 in Some Drugs Using 1, 2-Naphthoquine-4-Sulphonate (NQS)

Riyadh Radhi Al-Araji1( ), Muthana Saleh Mashkour1, Emad Abbas Jaffar Al-Mulla2( )
Department of Chemistry, Faculty of Science, University of Kufa, P.O. Box 21, An-Najaf 54001, Iraq
College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, 54003 Al-Kufa, Iraq
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Abstract

In this study, simple and sensitive spectrophotometric method for the determination of folic acid (VB9) in pharmaceutical formulations was reported. The proposed method was based on the reaction between folic acid and 1, 2-naphthoquine-4-sulphonate (NQS) at alkaline medium (pH 11) to form deep yellow product. Beer's law was obeyed in the range of 0.75-10.5 μg/mL of folic acid at maximum wavelength of 436 nm. Under optimized reaction conditions, linear regression equation of the calibration curve was y = 0.048 x + 0.038 (μg/mL) with a linear correlation coefficient of 0.9988. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.159 μg/mL and 0.531 μg/mL, respectively. The method was successfully applied to the determination of folic acid in pharmaceutical formulations.

Keywords

Folic acid (B9)SpectrophotometricPharmaceutical formulation1-2-naphthoquine-4-sulfonate (NQS)

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Nano Biomedicine and Engineering
Volume 9 Issue 3,
September 2017
Pages 208-213
DOI: 10.5101/nbe.v9i3.p208-213
Cite this article:
Al-Araji RR, Mashkour MS, Al-Mulla EAJ. Spectrophotometric Determination of Vitamin Folic Acid B9 in Some Drugs Using 1, 2-Naphthoquine-4-Sulphonate (NQS). Nano Biomedicine and Engineering, 2017, 9(3): 208-213. https://doi.org/10.5101/nbe.v9i3.p208-213

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Received: 19 July 2017
Accepted: 14 August 2017
Published: 15 September 2017
© 2017 Riyadh Radhi Al-Araji, Muthana Saleh Mashkour, and Emad Abbas Jaffar Al-Mulla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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