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Research Article | Open Access

Enhanced Peptide Delivery and Sustainable Release of Pleurocidin Using N-Succinyl Chitosan Nanoparticle

Giritharan Bupesh1,2( ), Chinnaiyah Amutha2,6, Sakthivel Vasanth1,2, Tharaumasivam Siva Vijayakumar2,5, Kannaiyah Pandian3( ), Vellingiri Balachandar4( ), Durai Rajan Gunasekaran1
R&D Wing, Central Research Laboratory, Sree Balaji Medical College & Hospital (SBMCH), BIHER-Bharath University, Chrompet, Chennai-600 044, India
Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
Department of Inorganic Chemistry, University of Madras, Guindy Campus, Tamil Nadu, India
Department of Human Genetics & Molecular Biology, Bharathiar University, Coimbatore-641046, Tamil Nadu, India
Department of Biotechnology, Srimath Andavar College, Srirangam, Tamil Nadu, India
Department of Animal behavior & Physiology, Madurai Kamaraj University, Madurai, Tamil Nadu, India
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Abstract

Chitosan is a natural polymer that can be modified into chemically different types. In order to imply biomedical applications, the chitosan dissolved in aqueous medium. Therefore, in the present study, functionally substituted chemical groups such as O-carboxymethyl chitosan (CMC), N-succinyl chitosan (NSC), quaternary ammonium chitosan (QAC) and tripoly phosphate chitosan (TPC) were synthesized. The peptide derived from Clarias batrachus was used as a drug that is successfully entrapped with NSC nanoparticles and were characterized by FTIR, AFM, SEM, Zeta sizer, fluorescent imaging and SDS-PAGE. In addition, antimicrobial activity was examined. The results of NSC obtained particle size (~64 nm) with highest solubility and 80% of antimicrobial activity against multidrug resistant pathogen Klebsiella pneumonia. Finally, the release profile indicates that the NSC nanoparticles deliver the peptide with 75% sustainable release and good availability up to 96 h. The current research was the first to report the characterization of peptide with NSC nanoparticles by SDS-PAGE, in which the antimicrobial activity was enhanced up to 80%, the particle sizes were obtained ~64 nm from SEM and AFM, and NSC-peptide exhibited 75% of sustainable release and good availability up to 96 h.

Keywords

EncapsulationChitosanPeptide deliveryAntimicrobial activityN-succinyl chitosanClarias batrachus

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Nano Biomedicine and Engineering
Volume 9 Issue 4,
December 2017
Pages 324-332
DOI: 10.5101/nbe.v9i4.p324-332
Cite this article:
Bupesh G, Amutha C, Vasanth S, et al. Enhanced Peptide Delivery and Sustainable Release of Pleurocidin Using N-Succinyl Chitosan Nanoparticle. Nano Biomedicine and Engineering, 2017, 9(4): 324-332. https://doi.org/10.5101/nbe.v9i4.p324-332

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Received: 29 November 2017
Accepted: 12 December 2017
Published: 29 December 2017
© Giritharan Bupesh, Chinnaiyah Amutha, Sakthivel Vasanth, Tharaumasivam Siva Vijayakumar, Kannaiyah Pandian, Vellingiri Balachandar, and Durai Rajan Gunasekaran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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