Research Progress on DNA-based Technologies for Adulteration Detection in Non-bovine Milk
(
), Abstract
In recent years, it has been often reported that non-nominal ingredients are found in milks from minor dairy species. Adulteration detection in milks from minor dairy species provides important technical support for market surveillance. DNA-based detection is a common and effective approach for food authentication, and also has advantages in milk adulteration detection. In order to provide a reference for the quality control of minor species milks, this article reviews the general aspects of minor species milks and the application of common DNA-based qualitative and quantitative techniques in adulteration detection in minor species milks in term of target gene selection, and their principles, sensitivities, application scenarios, advantages and limitations.
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References
WAJAHAT W, AZAD Z R A A, NAZIR S, et al. Real time-PCR coupled with melt curve analysis for detecting the authenticity of camel milk[J]. Journal of Food Science and Technology, 2022, 59(4): 1538-1548. DOI:10.1007/s13197-021-05164-8.
CEBALLOS L S, MORALES E R, ADARVE G D L T, et al. Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology[J]. Journal of Food Composition and Analysis, 2009, 22(4): 322-329. DOI:10.1016/j.jfca.2008.10.020.
HINZ K, O’CONNOR P M, HUPPERTZ T, et al. Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk[J]. The Journal of Dairy Research, 2012, 79(2): 185-191. DOI:10.1017/S0022029912000015.
PLATH A, KRAUSE I, EINSPANIER R. Species identification in dairy products by three different DNA-based techniques[J]. Zeitschrift für Lebensmitteluntersuchung und-Forschung A, 1997, 205(6): 437-441. DOI:10.1007/s002170050195.
VAITHIYANATHAN S, VISHNURAJ M R, NARENDER REDDY G, et al. Authentication of camel meat using species-specific PCR and PCR-RFLP[J]. Journal of Food Science and Technology, 2021, 58(10): 3882-3889. DOI:10.1007/s13197-020-04849-w.
LIAO J, LIU Y F, KU T, et al. Qualitative and quantitative identification of adulteration of milk powder using DNA extracted with a novel method[J]. Journal of Dairy Science, 2017, 100: 1657-1663. DOI:10.3168/jds.2016-11900.
GIGLIOTI R, POLLI H AZEVEDO B T, et al. Detection and quantification of adulteration in milk and dairy products: a novel and sentive qPCR-based method[J]. Food Chemistry: Moclecular Sciences, 2022(4): 100074. DOI:10.1016/j.fochms.2022.100074.
AGRIMONTI C, PIRONDINI A, MARMIROLI M, et al. A quadruplex PCR (qxPCR) assay for adulteration in dairy products[J]. Food Chemistry, 2015, 187(15): 58-64. DOI:10.1016/j.foodchem.2015.04.017.
WANG Zhiying, LI Tingting, YU Wenjie, et al. Determination of content of camel milk in adulterated milk samples by normalized realtime polymerase chain reaction system based on single-copy nuclear genes[J]. Journal of the Science of Food and Agriculture, 2020, 100(8): 3465-3470. DOI:10.1002/jsfa.10382.
GANOPOULOS I, SAKARIDIS I, ARGIRIOU A, et al. A novel closed-tube method based on high resolution melting (HRM) analysis for authenticity testing and quantitative detection in Greek PDO Feta cheese[J]. Food Chemistry, 2013, 141(2): 835-840. DOI:10.1016/j.foodchem.2013.02.130.
SAKARIDIS I, GANOPOULOS I, ARGIRIOU A, et al. High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo Mozzarella and other buffalo dairy products[J]. International Dairy Journal, 2013, 28(1): 32-35. DOI:10.1016/j.idairyj.2012.08.006.
PEREIRA L, GOMES S, BARRIAS S, et al. Applying high-resolution melting (HRM) technology to olive oil and wine authenticity[J]. Food Research International, 2018, 103: 170-181. DOI:10.1016/j.foodres.2017.10.026.
KALOGIANNI D P. DNA-based analytical methods for milk authentication[J]. Zeitschrift fur Lebensmittel-Untersuchung und-Forschung A, 2018, 244(5): 775-793. DOI:10.1007/s00217-017-3016-x.
REBECCA S, HUGGETT J F, BUSHELL C A, et al. Evaluation of digital PCR for absolute DNA quantification[J]. Analytical Chemistry, 2011, 83(17): 6474-6484. DOI:10.1021/ac103230c.
WANG Yi, WANG Yan, LAN Ruiting, et al. Multiple endonuclease restriction real-time loop-mediated isothermal amplification a novel analytically rapid, sensitive, multiplex loop-mediated isothermal amplification detection technique[J]. The Journal of Molecular Diagnostics, 2015, 17(4): 392-401. DOI:10.1016/j.jmoldx.2015.03.002.
KIM M J, KIM H Y. Direct duplex real-time loop mediated isothermal amplification assay for the simultaneous detection of cow and goat species origin of milk and yogurt products for field use[J]. Food Chemistry, 2018, 246: 26. DOI:10.1016/j.foodchem.2017.11.014.
WANG Yifan, YU Zhezhe, LIU Yongfeng. A high sensitivity method of closed-tube loop-mediated isothermal amplification developed for visual and rapid detection of cow milk adulteration[J]. International Dairy Journal, 2021, 127: 105214. DOI:10.1016/j.idairyj.2021.105214.
YU Wenjie, CHEN Yanjing, WANG Zhiying, et al. Multiple authentications of high-value milk by centrifugal microfluidic chip-based real-time fluorescent LAMP[J]. Food Chemistry, 2021, 351(3): 129348. DOI:10.1016/j.foodchem.2021.129348.
XIA Xuhan, YANG Hao, CAO Jijuan, et al. Isothermal nucleic acid amplification for food safety analysis[J]. Trends in Analytical Chemistry, 2022, 153: 116641. DOI:10.1016/j.trac.2022.116641.
EWIDA R M, EL-MAGIUD D S M A. Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism[J]. Veterinary World, 2018, 11(6): 830-833. DOI:10.14202/vetworld.2018.830-833.
JIANG Y B, WANG H, HOU D J, et al. Identification of bovine, buffalo and yak milk with polymerase chain reaction-restriction fragment length polymorphism[J]. Journal of Food Agriculture and Environment, 2013, 11(3): 455-457.
UNCU A O, UNCU A T. A barcode-DNA analysis method for the identification of plant oil adulteration in milk and dairy products[J]. Food Chemistry, 2020, 326: 126986. DOI:10.1016/j.foodchem.2020.126986.
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