Nanocrystalline hydroxyapatite has been extensively used for biomedical field, such as drug carrier, antitumor agent, surface coating, and bone-regenerating material. The wet chemical method is one of the most commonly used methods in the synthesis of nanosized hydroxyapatite due to its relatively low costs and more customizable control of the final product. Herein, we updated the most recent advances in artificial nano-hydroxyapatite prepared from biomimetic precipitation throughout context. Furthermore, micron-sized particles and macro-sized scaffolds made of nano-hydroxyapatite deposition are introduced. Their outstanding physicochemical properties and potential clinical application are highlighted. This article reviews the latest progress on preparing biomimetic nano-hydroxyapatite and provides inspiration to promote new breakthrough in regenerative therapy and clinical translation.