In this study, graft nano co-polymer was synthesized by esterification method of phthalic anhydride and glycerol. Nano polymer was diagnosed by infrared spectroscopy FT-IR, atomic force microscopy technique (AFM), X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC). Tendency of fabricated nano-polymer to adsorb Eosin yellow from aqueous solutions was evaluated. The average size of the height density of the nano-polymer was 22.04 nm. The effects of three different temperatures (298, 308 and 318 K) and concentrations (1, 3, 5 and 7 ppm) for nano polymer have been studied. The study's findings made it abundantly evident that manufactured nano-polymers were highly effective in completely removing Eosin yellow from aqueous solutions.