The unique properties of carbon quantum dots(CQDs) make them promising materials in many fields. Herein, we present a facile method for the preparation of photo-luminescent CQDs using humins as the carbon precursor for the purpose of providing a high value-added solution for this "biomass conversion process waste". The structure of the CQDs was analyzed, and the effects of reaction temperature and time on the CQDs' fluorescence were investigated. The results showed that humins were effectively carbonized during the reaction. The fluorescence intensity of humin-based CQDs initially increased with reaction temperature and time, and subsequently decreased beyond 200℃ and 4 h. Polyaromatic structures and hydrophilic groups such as O—H, C—O, —COOH and C＝O groups exist in the CQDs. The huminbased CQDs have the dimension of 3~7 nm with an average size of about 5.5 nm. The highest emission intensity of blue/cyan fluorescence light at 440 nm is achieved on the excitation with UV light at the wavelength of 330 nm.