In general, previous geothermal geochemical studies in Guangdong Province mainly involves single method to cover limited aspects and areas. In that way, various methods available cannot actually provide more convincing results of geothermal fluid’s circulation system and evolution process from different dimensions, especially in terms of isotope. As a result, more comprehensive researches remain to be done on geochemistry of geothermal fluid, in particular, the space-time law of isotope’s evolution pattern as well as recharge cycle. Based on data of environmental isotopes (²H and ¹⁸O) and the isotope of radiometric dating (¹⁴C), geothermal geology, characteristics of groundwater flow field and types of goethermal reservior in Guangdong Province are taken into account in this paper, so as to analyze numerical rule and spatial distribution features of isotopes. Thus, corresponding main causes, mechanism and hydrogeological significance can be revealed to further study the potential of geothermal fluid to renew and recharge in the long run, which is conducive to enrich geothermal theories and solve existing hydrogeological problems.