In this study, the biological effects of functionalized carbon nanotubes (CNTs) were investigated on cell morphology, proliferation, apoptosis, tissue pathology and blood test in vivo & in vitro. The functionalized CNTs had good biocompatibility at lower concentrations, and the functionalized single-walled carbon nanotubes(SWCNTs) perform in the early period in the animal body and multi-walled carbon nanotubes(MWCNTs) mainly in the late. The results show successful functional groups and no change in toxicity in functional samples compared with the primary sample, and there is a safety dosage on the normal cells and tissue. In subsequent studies, antitumoral investigations of modified samples will be evaluated.