As one of the most promising platforms for wireless communication, radiofrequency (RF) electronics have been widely advocated for the development of sensing systems. In particular, monolayer and few-layer two-dimensional (2D) materials exhibiting extraordinary electrical properties not only can be integrated to improve the performance of RF circuits, but also to display exceptional sensing capabilities. This review provides an in-depth perspective of current trends and challenges in the application of 2D materials for RF biochemical sensing, including: (ⅰ) theoretical bases to achieve different sensing schemes; (ⅱ) unique properties of 2D materials for reasoning their applications in RF sensing; (ⅲ) developments in 2D RF sensors to facilitate the practice of biochemical sensors with ever-demanding sensitivities, as well as their potential uses in meeting the requirements and challenges of biochemical sensors in the Internet-of-Things era.