In the present study, green synthesis of silver nanoparticles (AgNPs) were prepared by using Acalypha indica (AI) plant aqueous extract, which is used as a reducing and capping agent. The surface plasma resonance of AI-AgNPs was obtained at 436 nm by the ultraviolet–visible (UV–Vis) spectrum, which confirmed the formation of AI-AgNPs. Fourier transform infrared (FTIR) spectrometer studies revealed that phenolic and carbonyl groups are involved in the reduction of Ag⁺ to Ag. The transmission electron microscope (TEM) micrograph reveals that the size of green synthesized AI-AgNPs was obtained in the range of 18.7 with spherical morphology. The negative zeta potential of –16.1 mV of AI-AgNPs indicates the surface charge of the AI-AgNPs as negative the colloidal formulation is moderately stable. The current investigation additionally encompasses the demonstration of the potent antimicrobial efficacy of AI-AgNPs against Escherichia coli, Salmonella typhi, Staphylococcus aureus, and Streptococcus pyogenes. Furthermore, the study involves subjecting the green synthesized AI-AgNPs to assessments of antioxidant activity using, NO, and H₂O₂ methodologies, anti-cancer studies, DNA binding studies, and photocatalytic dye degradation.